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Application of Bepridil or KB-R7943 in preparation of a medicine for treating melanoma

A technology of KB-R7943 and melanoma, which is applied in the application field of medicine, and achieves the effect of wide application prospect, good curative effect and quick effect

Pending Publication Date: 2020-07-28
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the prior art, there is no report about the application of the compound Bepridil or KB-R7943 in the preparation of drugs for the treatment of melanoma

Method used

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  • Application of Bepridil or KB-R7943 in preparation of a medicine for treating melanoma
  • Application of Bepridil or KB-R7943 in preparation of a medicine for treating melanoma
  • Application of Bepridil or KB-R7943 in preparation of a medicine for treating melanoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Cell Experiment

[0040] 1 Experimental method

[0041] Human melanoma cell lines A2058 and A375 were cultured with DMEM medium + 10% fetal bovine serum. Cells were seeded in 96-well plates at 9000 cells / well, and cultured in a 37°C incubator containing 5% carbon dioxide. After the cells have grown to 70%-80%, a series of Bepridil or KB-R7943 of different concentrations are added to the culture medium. After the drug is incubated for 2 days, the death of the cells is measured, and the cell viability is measured by the CCK-8 method.

[0042] 2 results

[0043] The results showed that both Bepridil and KB-R7943 significantly inhibited the growth of melanoma cells. The dose-effect relationship of Bepridil in inhibiting tumor cell growth is as follows figure 2 As shown, Bepridil dose-dependently killed melanoma cell lines A2058 and A375 ( figure 2 ), the 50% inhibitory concentrations (IC50) produced by fitting the dose-response curve and repeating the experiment for 5...

Embodiment 2

[0048] Example 2 In vivo animal experiment

[0049] 1 Experimental method

[0050] 1) Cultivation of mouse melanoma cell line B16 and establishment of mouse subcutaneous implantation model:

[0051] Cultivate mouse melanoma cell line B16 with RPMI-1640 medium + 10% FBS, and place it at 37°C with 5% CO 2 Subculture growth in the incubator. Take logarithmic growth phase cells, wash with PBS and digest with 0.25% trypsin, collect cells with PBS to prepare cell suspension, trypan blue staining test identified cell survival rate greater than 92%, adjust the cell concentration to 100000 / μl after 30 minutes It is implanted into the rat brain, and the process of cell implantation is briefly described below.

[0052] The establishment of melanoma model: select male C57BL / 6J mice aged 8 months, use isoflurane anesthesia machine for general anesthesia, shave the mouse's right posterior axilla as the center, wipe and disinfect the left hand with alcohol cotton ball Lift the central skin with sh...

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Abstract

The invention relates to the technical field of biological medicines, and particularly relates to application of Bepridil or KB-R7943 in preparation of a medicine for treating melanoma. The inventionprovides a new strategy for resisting melanoma, namely the inhibitor Bepridil or KB-R7943 of a sodium-calcium exchanger is applied to treat melanoma, and belongs to new application of an old medicine.The mechanism of Bepridil or KB-R7943 for killing tumor cells is completely different from that of the conventional medicine, and drug resistance is not generated. Due to the use of the two new medicines, prognosis of a patient suffering from melanoma is expected to be improved, the survival rate of the patient is increased, the social burden is relieved, and bright prospect is brought to treatment of melanoma.

Description

Technical field [0001] The present invention relates to the technical field of biomedicine, specifically, the application of Bepridil or KB-R7943 in the preparation of medicines for treating melanoma. Background technique [0002] Melanoma is a malignant tumor that develops from melanocytes that originate in the nerve ridge. Most of them evolve from normal moles and pigmented spots, and they mostly occur on the skin. In addition, melanocytes also exist outside skin cells. For example, mucosal melanoma often occurs in the sinuses, oral cavity, anus, vulva, and vagina of the head and neck, and also occurs in the mucous membrane between the gastrointestinal tract and the genitourinary tract. 50% to 80% of patients with advanced melanoma will develop liver metastases, and 8% to 46% of patients with melanoma will develop brain metastases. The median survival time of metastatic advanced melanoma is only 8 to 9 months. Melanoma once Known as the "cancer king". [0003] Malignant melanom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/40A61K31/18A61K45/06A61P35/00
CPCA61K31/40A61K31/18A61K45/06A61P35/00A61K2300/00
Inventor 宋明柯
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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