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Bionic pre-vascularization material as well as preparation method and application thereof

A biomaterial and leaf vein technology, applied in the field of biomedicine, can solve the problems of long time, insufficient tissue thickness, toxicity, etc., and achieve the effect of improving the survival rate

Active Publication Date: 2020-07-28
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the biotechnology method needs to select a suitable allogeneic or heterogeneous tissue, and use chemical or physical methods to decellularize to obtain matrix materials. The whole process is relatively complicated and time-consuming, and it involves ethical and rejection issues in clinical applications. ; In vitro culture of cell membranes requires the technology and platform for preparing temperature-sensitive materials. In vitro culture of 3D vascularized materials with sufficient tissue volume requires multi-layer membrane superposition, which has relatively complicated technical means and many operating procedures; Materials involve the modification of materials, microstructure, tissue description, degradation rate and toxicity of degradation products, etc.
The way of skin tissue mixed cell culture, the cycle of cell extraction and culture is long, the thickness of the obtained tissue is not enough, it is not suitable for large-scale skin or tissue repair, etc.

Method used

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  • Bionic pre-vascularization material as well as preparation method and application thereof
  • Bionic pre-vascularization material as well as preparation method and application thereof
  • Bionic pre-vascularization material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] A method for preparing a bionic prevascularized material, comprising the following steps:

[0079] Step 1. Fabrication of vein structure: Select leaves with reticular veins, which are moderately mature, with light yellow mesophyll and yellowish-brown veins. Put the leaves in 10% sodium hydroxide solution and boil for 10 minutes, wash off the residual sodium hydroxide solution with running water, scrape lightly with tweezers or brush off the mesophyll, and keep the complete leaf vein structure, such as figure 1 shown;

[0080] Place the leaf vein structure in a petri dish or other container, moisten it so that it completely fits the bottom of the container; leaf vein laying method: the back of the leaf vein structure faces up (that is, the back of the leaf faces up), and the other side of the leaf vein structure faces the culture Dish;

[0081] Step 2. PDMS film production: use commercially available Dow Corning SYLGARD™ 184 silicone elastomer and make PDMS according t...

Embodiment 2

[0094] A method for preparing a bionic prevascularized material, comprising the following steps:

[0095] Step 1. Fabrication of vein structure: Select leaves with reticular veins, which are moderately mature, with light yellow mesophyll and yellowish-brown veins. Boil the leaves in water for 5 minutes, wash away the residual solution with running water, gently scrape with tweezers or brush to remove the mesophyll, after cleaning, the leaf veins can be obtained, and the complete leaf vein structure is preserved, and it is ready to use after cleaning;

[0096] Place the vein structure in a petri dish or other container, moisten it so that it is completely attached to the bottom of the container; the method of laying the vein: the back of the vein structure faces upward, and the other side of the vein structure faces the petri dish;

[0097] Step 2. PDMS film production: use commercially available Dow Corning SYLGARD™ 184 silicone elastomer and make PDMS according to its instruc...

Embodiment 3

[0108] A method for preparing a bionic prevascularized material, comprising the following steps:

[0109] Step 1. Place the vein structure purchased in the market in a petri dish or other container, moisten it so that it fits completely with the bottom of the container; the method of laying the vein: the back of the vein structure faces upward, and the other side of the vein structure faces the culture Dish;

[0110] Step 2. PDMS film production Use the commercially available Dow Corning SYLGARD™ 184 silicone elastomer and make PDMS according to its instructions. Mix the silicone and curing agent at a ratio of 12:1. After mixing, centrifuge at 3000rpm / min for 5 minutes; Pour the centrifuged PDMS into a petri dish to cover the leaf veins in step 1, with a thickness of about 0.1mm, and place it on the table, vacuumize to reduce air bubbles; react at 100°C for 8 hours and the PDMS is cured. After returning to room temperature, the veins were removed to obtain a PDMS film with a ...

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Abstract

The invention relates to the technical field of biological medicines, and discloses a bionic pre-vascularization material as well as a preparation method and application thereof. The preparation method of the pre-vascularization material comprises the steps of preparing a biological material with a vein structure, performing in-vitro cell culture and performing in-vivo transplantation. The pre-vascularization material can be applied to preparation of a wound repair material, a scaffold material, an organ reconstruction material, a tissue replacement material, a tissue filling material or a wrapping material and the like. A vein network structure of a pre-lymphatic vascularization material is similar to a lymphatic network structure of the human body; and a mesh branch structure of the leafvein of the pre-vascularization material is similar to a microvascular network structure of the human body. The bionic pre-vascularization material has the mechanical properties suitable for expectable application and can keep sufficient integrity until tissues grow inwards and heal; and the bionic pre-vascularization material has the characteristic of being easily processed and shaped into a product and the acceptable storage life, but does not cause significant inflammatory or toxic response in vivo.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and more specifically, to a bionic prevascularized material and its preparation method and application. Background technique [0002] Vascularization is a necessary condition for wound repair. Early vascularization can improve graft survival, wound repair efficiency and quality. It has been proven that prevascularized materials can establish blood circulation 3 days earlier than non-prevascularized materials. It can be seen that the preparation and application of prevascularized materials has a strong necessity and good application effect. [0003] Lymphatic edema and tissue fibrosis caused by lymphatic damage and dysfunction caused by various reasons seriously affect the quality of life of patients. However, methods and means for repairing lymphatic vessel damage and improving lymphatic function have not been significantly improved. Therefore, it is necessary and important to explore a wa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/38A61L27/50
CPCA61L27/222A61L27/3804A61L27/3808A61L27/50A61L27/507A61L2430/22
Inventor 张利军邢孟秋吴军
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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