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Method for simultaneously extracting inulin and total caffeoylquinic acid from stevia rebaudiana roots

A technology of caffeoylquinnic acid and stevia, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as unmeasured content

Pending Publication Date: 2020-07-28
巴州正达绿源生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in the literature that there is inulin contained in the hairy root culture system of Stevia rebaudiana, but the content was not determined and the corresponding extraction and utilization methods were not proposed (Sheila M.S. Lopes, Gabriela Krausová, Vojtěch Rada, José E. Regina A.C. Arildo J.B.de Oliveira, Isolation and characterization of inulin with a high degree of polymerization from roots of Stevia rebaudiana (Bert.) Bertoni[J].Carbohydrate Research,2015,411)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Take 100kg stevia root for impurity removal and cleaning, and use normal temperature water extraction as it is or chopped, the solid-liquid ratio is 1:30, after solid-liquid separation, stevia root extract is obtained, and 15.7kg of inulin and The extract of total caffeoylquinic acid 3.2kg.

[0031] The obtained stevia root extract was removed, extracted and separated with ethyl acetate to obtain 2.5 kg of total caffeoylquinic acid, and the raffinate was filtered to obtain 14.5 kg of inulin.

Embodiment 2

[0033] Take 100kg stevia root for impurity removal and cleaning, use 30°C and 40% ethanol extraction as it is or chopped, stevia root:solvent solid-liquid ratio is 1:60, obtain stevia root extract after solid-liquid separation Fine filtration and separation obtained 10.3 kg of inulin, and 4.2 kg of total caffeoylquinic acid was obtained through macroporous resin adsorption separation.

Embodiment 3

[0035] Take 100kg stevia root to remove impurities and wash it, and use 60°C and 30% methanol to extract as it is or chopped. The stevia root:solvent solid-to-liquid ratio is 1:10. Obtain inulin 15.9kg and total caffeoylquinic acid 3.3kg simultaneously.

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PUM

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Abstract

The invention provides a method for simultaneously extracting inulin and total caffeoylquinic acid from stevia rebaudiana roots. Steps of digestion, fermentation, extraction, enzyme treatment, resin separation and the like are used in the method. According to the method disclosed by the invention, the stevia rebaudiana root waste in stevia rebaudiana planting and stevioside production processes isused for extracting or fermenting various high-added-value products, so that the method has good economic benefits, environmental benefits and social benefits.

Description

technical field [0001] The invention belongs to the field of deep processing of plants produced by plant extracts. Specifically, the invention provides a method for simultaneously extracting inulin and total caffeoylquinic acid from stevia roots Background technique [0002] Inulin (also known as inulin) is a polysaccharide composed of fructose polymerization. It is low in calories and non-toxic. It can prevent dental caries, regulate lipid metabolism, promote calcium absorption, reduce serum cholesterol, suppress cancer and act as a bifidobacteria proliferation factor. The health effect of improving human immunity: due to the function of water-soluble dietary fiber, it can be used as a rheology and tissue modifier, water retention agent, etc. Widely used in low-calorie beverages, low-fat or non-fat spreads, yogurt, ice cream, chocolate and other foods (Klont R, Mannion P.Inulin&oligofructose[J].World of Ingredients.1999.(9):34-36) . [0003] Inulin is widely distributed i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/18C07C69/732C07C67/48C07C67/56C12P19/04C12P19/00C12P7/42C12P7/56C12P7/06A23K10/12A23K10/30C12R1/685C12R1/865C12R1/845
CPCC08B37/0003C08B37/0054C07C67/48C07C67/56C12P19/04C12P19/00C12P7/42C12P7/56C12P7/06A23K10/12A23K10/30C07C69/732Y02E50/10
Inventor 陈育如王屹东王艳羽王雪铭张红艺李德梅程致远
Owner 巴州正达绿源生物科技有限公司
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