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A method for promoting the improvement of fish growth traits

A growth trait and growth-promoting technology, applied in the fields of genetic breeding and genetic engineering, can solve problems such as unclear relationships, and achieve the effect of significant aquatic breeding value

Active Publication Date: 2022-02-15
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the genetic mechanism leading to the excellent growth traits of Zhongke-3 allogenetic gibel carp and its relationship with the HPI axis is still unclear.

Method used

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  • A method for promoting the improvement of fish growth traits
  • A method for promoting the improvement of fish growth traits
  • A method for promoting the improvement of fish growth traits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Construction of pomc-deleted zebrafish mutants using gene editing

[0044] 1. Application of TALENs technology to knock out the zebrafish pomc gene

[0045] (1) Design and assembly of TALENs target sites

[0046] According to the basic principles of TALENs (transcription activator-like effector nucleases, transcription activator-like effector nucleases) design (Cermak et al., 2011) and the gene sequence information of zebrafish pomca (Gene ID: 353221) in the NCBI gene bank , designing targeting arms on both sides of the pomca gene target site. To obtain mutant M1 that lacks all POMC-derived polypeptide hormones, the target site was designed for frameshift mutation after the translation initiation site ATG and before the sequence encoding α-MSH. The assembly of the TALENs arm was performed in strict accordance with the instructions provided by Addgene's GoldenGate TALENs Kit kit. The specific sequence of the selected target site is: left arm: 5'-GTTGGGAAAATGCCCGCT-3';...

Embodiment 2

[0101] Association Analysis of HPI Axis Signal Molecule Gene Polymorphisms and Growth Traits of Crucian Carp

[0102] 1.2b-RAD sequencing and association analysis of feed conversion traits

[0103] In the present invention, the female fish of Dongting crucian carp is mated with the male Zhongke No. 3 fish to obtain F1 generation hybrid fish, and 57 hybrid fish with similar body weight are selected for a 60-day FCE experiment. Before and after the FCE experiment, the average body weight of the hybrid fish was 62.66g and 87.95g, respectively. The average value of FCE of F1 generation hybrid fish is 35.20% ( Figure 13 ). The genomes of F1 generation hybrid fish were extracted and subjected to 2b-RAD sequencing and association analysis of feed conversion ratio traits. After filtering out some low-quality and non-BcgⅠ restriction site reads, a total of 111.61 million reads were obtained, with an average of 1.958 million reads per sample. In the parents of Dongting crucian carp...

Embodiment 3

[0111] Molecular marker kits for growth-enhancing breeding in fish

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Abstract

The invention discloses a method for promoting the improvement of fish growth traits. The present invention specifically relates to knocking out genes related to signaling molecules of the fish hypothalamus-pituitary-adrenal axis pathway by gene editing, thereby inhibiting the hypothalamus-pituitary-adrenal axis signaling pathway, thereby promoting the growth of fish. The present invention obtains the genotype of the signal molecule that promotes fish growth in the hypothalamus-pituitary-adrenal axis pathway through the association analysis of the gene polymorphism of the fish hypothalamus-pituitary-adrenal axis signal molecule and the growth traits of the fish, As an auxiliary molecular marker for fish genetic breeding improvement, it can promote the genetic improvement of fish growth traits.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to the gene editing of fish hypothalamus-pituitary-adrenal axis signaling pathway signal molecules, and inhibiting the fish hypothalamus-pituitary-adrenal axis signaling pathway to promote the growth of individual fish; it also relates to genetic In the field of breeding, it specifically relates to the molecular marker technology for genetic improvement of fish growth-promoting traits. Background technique [0002] The growth of individual fish is an important economic trait in aquaculture. The growth rate of fish can be accelerated and the feed conversion rate can be improved through genetic assisted breeding technology. This directional breeding technology can directly increase fishery output, reduce feed consumption, and achieve the economic purpose of ecological farming. The growth process of individual animals is a regulatory process of energy and material metabolism. The ...

Claims

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Application Information

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IPC IPC(8): C12N15/90A01K67/027C12Q1/6888
CPCC12N15/902A01K67/0276C07K14/68C12Q1/6888C12Q2600/156C12Q2600/124C12Q2600/158A01K2217/075A01K2227/40A01K2267/02
Inventor 殷战石闯童金苟解绶启娄气永翟刚
Owner INST OF AQUATIC LIFE ACAD SINICA
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