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Method for inhibiting growth and metastasis of solid tumor cells and special pharmaceutical composition

A technology of tumor cells and compositions, applied in the field of animal cell proliferation and transformation

Active Publication Date: 2020-07-31
TIANJIN PEOPLE HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research work on the induction of solid tumor differentiation is still in the application stage. The application of certain chemical substances can reverse the immature malignant cells and differentiate into normal cells.

Method used

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  • Method for inhibiting growth and metastasis of solid tumor cells and special pharmaceutical composition
  • Method for inhibiting growth and metastasis of solid tumor cells and special pharmaceutical composition
  • Method for inhibiting growth and metastasis of solid tumor cells and special pharmaceutical composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] ① Malignant tumor cells are ovarian cancer HEY cell line and breast cancer MDA-MB-231 cell line; the method of inducing fat differentiation of HEY cells and MDA-MB-231 cells in vitro is as follows:

[0072] with high concentration CoCl 2 Induce two cell lines to obtain cells with high invasion and metastasis ability: the ovarian cancer cell line HEY, use RPMI-1640 medium 5mL plus 535.5μL CoCl 2 Replace the RPMI-1640 medium after 48 hours of action; the breast cancer cell line MDA-MB-231 uses DMEM medium 5mL + 535.5μL CoCl 2 , after 33 hours of action, replace the DMEM medium; observe the state of the cells, replace the fresh medium, and after the cells recover for 10-14 days, when the CoCl 2 When the treated cells recover to 80%-90% confluence, use the same concentration of CoCl again 2 Treated for the same time, after a high concentration of CoCl 2 After repeated treatment twice, a sufficient number of cells was obtained.

[0073] ②The control group cells induced a...

Embodiment 2

[0085] Construction of xenograft tumor model in nude mice

[0086] The control cells induced and differentiated in vitro and CoCl 2 Induced cells were subcutaneously injected into the left groin of 6-8-week-old Balb / c immunodeficient female mice to establish a mouse xenograft tumor model. Mouse xenograft tumor experiments confirmed that the adipocytes obtained from in vitro induction of ovarian cancer HEY cell line and breast cancer MDA-MB-231 cell line had normal adipocyte-specific molecular labels and existed stably in the mouse xenograft tumor model.

[0087] When constructing the xenograft tumor model in nude mice, 20 BALB / cNU / NU nude mice (Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.), all 6-8 weeks old, female, were used to acclimatize in the SPF level nude mouse breeding room for 1 5 rats / cage, 6 cages in total, change the mouse cage once a week during the feeding period, pay attention to the sterilization of feed, litter, water source and mouse cage;...

Embodiment 3

[0106] The final concentration and therapeutic dose of the pharmaceutical composition (DRI) for animal experiments:

[0107] (1) On the 21st day of the tumor formation experiment in nude mice in each group, the tumor volume of each group exceeded 100 mm 3 After that, the HEY cell tumorigenesis group, CoCl 2 The treated cell groups were randomly divided into a drug composition (DRI) treatment group, a DMSO control group, and a blank control group.

[0108] (2) Explore the different drug treatment doses in the DRI treatment group: according to the LD50, IC50, and EC50 of the three small molecule compounds, the preferred three drug treatment doses are: dexamethasone (10mM×1mL dissolved in DMSO) treatment dose: 1.77- 4.17μg / 25g mouse body weight; Rosiglitazone (10mM×1mL dissolved in DMSO) therapeutic dose: 400-625μg / 25g mouse body weight; 3-isobutyl-1-methylxanthine (10mM×1mL dissolved in DMSO) therapeutic dose: 180.57-275 μg / 25g mouse body weight.

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Abstract

The invention discloses a method for inhibiting the growth and metastasis of solid tumor cells and a special pharmaceutical composition, and belongs to the field of animal cell proliferation and transformation. When ovarian cancer HEY cells or breast cancer MDA-MB-231 cells are induced, a culture medium is replaced after acting in high-concentration CoCl2, and after the cells are recovered, the treatment is repeated twice to obtain a sufficient number of cells with high invasion and metastasis ability; and the obtained cells are cultured in a culture medium without CoCl2, then the removed culture medium is added with a pharmaceutical composition prepared from dimethyl sulfoxide, dexamethasone, rosiglitazone, 3-isobutyl-1-methylxanthine for in-vitro induction, and when obvious lipid droplets are formed in tumor cell plasma, it indicates that the tumor cells have adipose differentiation. The method is designed to induce the tumor cell adipose differentiation and the purpose of inhibitingtumor cell growth is achieved. It is confirmed by animal experiments that the method is expected to be used for clinical treatment and metastasis inhibition of human solid tumors.

Description

technical field [0001] The invention relates to the proliferation and transformation of animal cells, in particular to a method for inhibiting the growth and metastasis of solid tumor cells and a special pharmaceutical composition. Background technique [0002] Tumor is a systemic disease with the highest morbidity and mortality in humans. At present, the main methods for the prevention and treatment of tumors include: surgery, radiotherapy and chemotherapy. Compared with surgical treatment and radiotherapy, drug treatment is systemic effect therapy. Traditional chemotherapy drugs not only kill tumor cells, but also have a killing effect on normal body cells. Their toxicity and drug resistance cannot be ignored. In addition, chemotherapeutic drugs can also enhance the ability of tumor cells to invade and metastasize, and the tolerance of tumor cells to chemotherapeutic drugs is enhanced, which eventually leads to tumor recurrence and metastasis. The most significant biolog...

Claims

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Application Information

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IPC IPC(8): A61K31/4439A61K31/573A61K31/522A61P35/00C12N5/09
CPCA61K31/4439A61K31/573A61K31/522A61P35/00C12N5/0693A61K2300/00
Inventor 张诗武张可昕李玉玮
Owner TIANJIN PEOPLE HOSPITAL
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