Reverse genetic system for rescuing PEDV (porcine epidemic diarrhea virus) ZJU/G2/2013 strain based on DNA (deoxyribonucleic acid) plasmid transfection
A technology of plasmid transfection and reverse genetics, which is applied in the direction of recombinant DNA technology, positive-sense single-stranded RNA virus, and microbial detection/inspection, etc. problem, to achieve the effect of improving rescue efficiency, strong application value, and easy detection
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0043] 1. Amplification of the target fragment of PEDV-G2-ZJU-GLuc full-length infectious clone
[0044] According to the gene sequence of the porcine epidemic diarrhea virus G2 gene subtype ZJU / G2 / 2013 strain published on Genbank by the inventor (the Genbank number is KU558701.1, the gene structure is as follows figure 1 ), the full length of the viral genome is 28038nt. Take the cell infection supernatant sample of the cell stable passage strain ZJU / G2 / 2013 strain (source: Zhejiang virulent strain isolated by the inventor in 2013), and extract RNA by Trizol method to obtain cDNA by reverse transcription. 5 pairs of primers (see Table 2 for the primer sequences) were amplified to obtain the corresponding 5 genome full-length fragments, cloned through the pCR-Blunt vector, picked 3 monoclonal colonies, extracted the plasmids for sequencing and spliced the sequencing results to determine the ZJU / The full-length genome sequence of G2 / 2013.
[0045] Using 5 pairs of primers...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com