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Cell-penetrating peptide modified drug-loaded thermosensitive nanoparticle and application thereof in resisting melanoma

A technology of nanoparticles and membrane-penetrating peptides, which is applied in the field of nanobiomedicine and can solve the problems of slow drug release and leakage

Inactive Publication Date: 2020-08-04
XUZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past ten years, the nano-delivery system loaded with chemotherapy drugs has made great progress in anti-tumor therapy, but the effect of single treatment mode is not ideal, because the accumulation of nanoparticles in tumor tissue does not necessarily lead to higher intratumoral drug concentration, drug release from nanoparticles is a slow phenomenon that relies on passive leakage

Method used

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  • Cell-penetrating peptide modified drug-loaded thermosensitive nanoparticle and application thereof in resisting melanoma
  • Cell-penetrating peptide modified drug-loaded thermosensitive nanoparticle and application thereof in resisting melanoma
  • Cell-penetrating peptide modified drug-loaded thermosensitive nanoparticle and application thereof in resisting melanoma

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Embodiment 1

[0028] Example 1: Study on the Targeting Effect of Drug-loaded Thermosensitive Nanoparticles Modified by Membrane-penetrating Peptides on Melanoma

[0029] 1 material

[0030] 1.1 Reagents

[0031]

[0032]

[0033] 1.2 Instruments

[0034]

[0035]

[0036] 2 methods

[0037] 2.1 Preparation of TAT-TSL-IR820-TMZ nanoparticles

[0038] 2.1.1. Synthesis of DSPE-PEG2000-TAT

[0039] Dissolve 20mg of TAT and 15mg of DSPE-PEG2000-Mal in 5ml of pure water, and stir gently at room temperature for 24 hours under the protection of nitrogen. The progress of the reaction was monitored by thin layer chromatography. After the reaction, it was placed in a dialysis bag (8000-14000Da) for 48 hours of deionized water dialysis, and then it was freeze-dried for use, that is, DSPE-PEG2000-TAT.

[0040] 2.1.2 TAT-TSL-TMZ / IR820 nanoparticles were prepared by thin film dispersion method.

[0041] Add 64mg DPPC, 16mg cholesterol, 4mg DSPE-PEG2000, 4mg DSPE-PEG2000-TAT, 5mg TMZ, 0....

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Abstract

The invention provides cell-penetrating peptide modified drug-loaded thermosensitive nanoparticles and application thereof in resisting melanoma, and belongs to the technical field of nano biomedicine. According to the cell-penetrating peptide modified drug-loaded thermosensitive nanoparticles, TSL is used as a carrier, and under the modification of cell-penetrating peptide TAT, the chemotherapy effect of TMZ and the PTT treatment effect of IR820 are combined to construct a novel thermosensitive nanoparticle TAT-TSL-IR820-TMZ with a synergistic anti-tumor effect, so that targeted delivery of treatment components is realized. In-vitro experiments show that the nanoparticles can be massively taken by melanoma cells, can target lysosome, and can play a role in combined chemotherapy and photothermal action in combination with NIR.

Description

technical field [0001] The invention belongs to the technical field of nano-biomedicine, and in particular relates to a drug-loaded thermosensitive nanoparticle modified by a membrane-penetrating peptide and its anti-melanoma application. Background technique [0002] Melanoma is a malignant tumor originating from melanocytes deep in the epidermis, and usually develops from deteriorating single melanocytes or benign dysfunctional moles. While rates of many other types of cancer have been declining in recent years, rates of melanoma have continued to rise. Melanoma is a skin tumor with strong invasiveness, high lethality and unpredictable course, and it tends to be younger. Currently, surgery, chemotherapy, and radiotherapy are still the main treatments for melanoma. In addition, immunotherapy and molecular targeted therapy based on immune checkpoint inhibitors have brought new hope to melanoma patients, but they have not significantly improved the overall survival rate in ...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/42A61K41/00A61K31/4188A61P35/00
CPCA61K9/5169A61K31/4188A61K41/0052A61K41/0057A61P35/00A61K2300/00
Inventor 蒋冠杨春生李欣欣侯晓阳王云
Owner XUZHOU MEDICAL UNIV
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