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Application of probiotic in preparation of preparation for treating liver diseases

A technology of liver disease and probiotics, which is applied in the application field of probiotics in the preparation of preparations for the treatment of liver diseases, and can solve the problems of lack of treatment methods for hepatic endoplasmic reticulum stress diseases

Active Publication Date: 2020-08-07
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no effective treatment for hepatic ER stress disease at present, so it is of great significance to seek a new and effective method for the treatment of hepatic ER stress disease

Method used

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  • Application of probiotic in preparation of preparation for treating liver diseases
  • Application of probiotic in preparation of preparation for treating liver diseases
  • Application of probiotic in preparation of preparation for treating liver diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] First, strain pretreatment

[0050] 1. Inoculate Bifidobacterium breve BNCC164835, Lactobacillus plantarum BNCC17465 and Lactobacillus plantarum BNCC162960 in the corresponding liquid medium on the ultra-clean workbench.

[0051] 2. Dissolve the Suzhou and American probiotics in 10 mL of MRS medium, and freeze in aliquots (500 microliters of 50% glycerol + 500 microliters of bacterial suspension). One tube of cryopreserved strains was inoculated into 50 mL of MRS liquid medium for each culture.

[0052] 3. Place the inoculated medium in a constant temperature incubator at 30°C for static culture.

[0053] 4. Take out the bacterial solution when it grows to OD600=1.2, centrifuge for 20 minutes at 4°C and 5000g.

[0054] 5. Discard the supernatant on the ultra-clean workbench, resuspend the pellet with sterile PBS, and centrifuge again at 4°C and 5000g for 20 minutes.

[0055] 6. Discard the supernatant pellet and resuspend with 30 mL of sterile PBS.

[0056] 7. Calcu...

Embodiment 2

[0063] According to the method of Example 1, the body weight of each group of mice was recorded once before the start of the experiment, and the body weight of the mice was recorded once a week after the start of the experiment until the end of the sixteenth week of the experiment to test the changes in the body weight and food intake of the mice.

[0064] Liver ERS mice were treated with probiotics since the age of two months, and lasted for four months. The weight changes of mice are shown in Table 2. When the mice were two months old, compared with the WT PBS group, the weight of the mice in the KO PBS group decreased significantly (P0.05). ); when the mice were six months old, compared with the WT PBS group, the body weight of the mice in the KO PBS group decreased significantly (P0.05); in terms of body weight gain, compared with the WT PBS group, the body weight gain of the KO PBS group was significantly reduced (P0.05).

[0065] Table 2 Effects of probiotics on body wei...

Embodiment 3

[0068] Following the method of Example 1, the collection of mouse samples was performed sixteen weeks after the experiment was performed.

[0069] 1. Serum acquisition: add 10 microliters of 1 mg / mL heparin sodium to a 1.5 mL centrifuge tube to collect fresh mouse blood in the above centrifuge tube. Centrifuge at 4°C and 12000g for 10 minutes, and carefully draw the upper layer liquid with a small-volume pipette to obtain serum. Freeze at -80 for later use.

[0070] 2. Tissue acquisition: Mice were sacrificed by vertebral dislocation, and the liver, spleen, peripheral lymph and intestinal lymph of the mice were dissected.

[0071] The liver weight ratio of mice is often used as an important indicator for judging liver damage. After probiotic intervention was performed on the liver ERS mice and the samples were collected, the liver weight and final body weight of the mice were weighed, and the liver weight ratio was calculated, where the liver weight ratio=mouse liver weight / ...

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Abstract

The invention relates to an application of a probiotic composition in preparation of a preparation for treating liver diseases. The probiotic composition comprises bifidobacterium breve and lactobacillus plantarum. The invention discloses the novel application of the probiotic composition, and provides a direction for developing the novel preparation for treating liver diseases.

Description

technical field [0001] The invention relates to the field of preparations for the treatment of liver diseases, in particular to the application of a probiotic in the preparation of preparations for the treatment of liver diseases. Background technique [0002] The liver is an important metabolic organ of the human body, and its main functions include the synthesis and decomposition of glucogen, the synthesis of plasma proteins, the lysis of red blood cells, the synthesis and secretion of bile, and the degradation of various metabolic intermediates. Due to the induction of genetic or external factors (overnutrition, alcohol or drug poisoning, viral infection), a variety of liver diseases can occur, including alcoholic liver injury, non-alcoholic fatty liver disease, liver fibrosis, liver failure, liver cirrhosis and liver cancer. The causes of liver disease are complex, the number of morbidity is large and the fatality rate is high. However, the current clinical intervention...

Claims

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Application Information

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IPC IPC(8): A61K35/745A61K35/747A61P1/16A61P35/00
CPCA61K35/745A61K35/747A61P1/16A61P35/00
Inventor 龙乔明龙光宇
Owner SUZHOU UNIV
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