Preparation method of animal decellularized lung biological scaffold material

A decellularization and bio-scaffold technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve problems such as unstable effect, low decellularization efficiency, and large damage to extracellular matrix components, and achieve enhanced tolerance , weaken the effect of contradiction

Active Publication Date: 2020-08-07
WEST CHINA HOSPITAL SICHUAN UNIV
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the current methods for obtaining decellularized lung bioscaffold materials at home and abroad are mainly suitable for small animals such as rats, and when applied to large animals, the decellularization efficiency is low, the effect is unstable, or the extracellular matrix components are damaged (refer to Literature Petersen TH, Calle EA, Zhao L, Lee EJ, Gui L, Raredon MB, Gavrilov K, Y

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of animal decellularized lung biological scaffold material
  • Preparation method of animal decellularized lung biological scaffold material
  • Preparation method of animal decellularized lung biological scaffold material

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0042] Example 1 Preparation of decellularized pig lung biological scaffold material

[0043] The animals were anesthetized with Shutai, and the lungs were taken out after whole-body heparinization. Insert and fix the catheter from the pulmonary artery and trachea. Infuse 2L of PBS solution containing heparin (the dose is sufficient for anticoagulation) from the cannula of the pulmonary artery (it can also be other isotonic solutions containing anticoagulant substances, such as: aqueous solution containing calcium ion chelating agent, low molecular weight heparin) , Heparin analogs, etc.), the perfusion time is 0.5 hours, and then placed in the refrigerator at -20°C for at least 24 hours. After the pig lungs are fully thawed, infuse 0.9% m / v saline and 0.2% m / v dextrose solution (osmotic pressure 10mOsm / L) successively from the trachea, the flow rate is 100 mL / min, and the perfusion time is 1 hour and 3, respectively. hour. Afterwards, 1% v / v SLES (other ionic detergents) aque...

Example Embodiment

[0052] Experimental example 1 Comparison of pig lung bioscaffold materials with and without dextrose solution perfusion treatment

[0053] This experimental example is based on Example 1, selectively omitting the step of treating the ECM protective agent dextrose, as a control, and compares the difference with the biological scaffold material obtained in Example 1.

[0054] The result is Figure 4-Figure 9 Shown:

[0055] Figure 4 It is a decellularized pig lung bioscaffold material that has been perfused with dextrose solution ( Figure 4 A) and pig lung bio-scaffold materials that have not been perfused with dextrose solution ( Figure 4 B). The results show that the decellularized pig lung bio-scaffold material perfused with dextrose solution has a firmer texture and better maintains the original organ shape.

[0056] Figure 5 with Image 6 The results show that the collagen and glycosaminoglycan content of the pig lung bioscaffold material after and without the dextrose solution...

Example Embodiment

[0059] Experimental example 2 Comparison of degradation performance and immunogenicity

[0060] In order to test the degradation performance and immunogenicity of the bio-scaffold material, the pig lung bio-scaffold material, which has been perfused with and without dextrose solution, was implanted into the rat omentum. At the 1st and 4th week of implantation, the material was taken out and fixed with 4% formalin, and stained after sectioning.

[0061] The result is Picture 9 It shows that the extracellular matrix components of the decellularized pig lung bioscaffold material perfused with dextrose solution degrade more slowly, and the number of neutrophils and lymphocytes infiltrating around the scaffold material is less, which causes the immunogen The sex is lower.

[0062] Conclusion: The decellularized pig lung bio-scaffold material treated with dextrose solution perfusion degrades slowly in animals, and can provide a more stable and long-lasting scaffold for stem cell attachme...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method of an animal decellularized lung biological scaffold material, and belongs to the field of tissue engineering and regenerative medicine. According to a traditional decellularized lung biological scaffold material, cells are removed by pouring a detergent, extracellular matrix (ECM) can be damaged while the detergent removes the cells, and then the scaffold structure is affected. According to the method disclosed by the invention, the ECM protective agents such as a dextrose solution are creatively used for filling the trachea, so that the damage ofthe detergent to the ECM is reduced, the contradiction between ECM retention and cell component removal is more easily balanced, and a stent material with more thorough cell removal and more intact ECM is obtained.

Description

technical field [0001] The invention belongs to the field of tissue engineering and regenerative medicine. Background technique [0002] Pulmonary failure caused by chronic obstructive pneumonia, pulmonary fibrosis and other diseases has become a major problem in the medical field. So far, lung transplantation is the only effective radical cure in clinical practice, but its development is restricted by factors such as shortage of donor lungs, difficulty in storing donor lungs during transport, and complicated surgical procedures. As a potential alternative treatment for orthotopic organ transplantation, tissue engineered organ technology has the advantages of diverse sources and simple treatment methods, and is gradually being valued. [0003] A number of studies have shown that biological scaffolds play a key role in supporting the distribution, differentiation, regeneration, and maintenance of cell functions, and are one of the cores of research in the fields of tissue en...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3633A61L27/3687A61L27/3691A61L27/50A61L2430/40
Inventor 李奕李为民
Owner WEST CHINA HOSPITAL SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap