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Method for enriching and purifying mangiferin from gentiana rhodantha and detection method thereof

A technology of safflower gentian and mangiferin, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve problems such as unfavorable development and utilization of safflower gentian medicinal materials.

Pending Publication Date: 2020-08-07
贵州中医药大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because there is no research report about the adsorption-analysis behavior of mangiferin in gentian safflower on the macroporous adsorption resin, it is not conducive to the further development and utilization of gentian safflower medicinal material. In view of this, the team of the present invention adopts an orthogonal test Design and macroporous resin adsorption purification technology, optimize mangiferin dynamic adsorption and desorption purification process parameters, use HPLC method as detection method, discuss the effect of D101 macroporous adsorption resin on the purification effect of mangiferin, in order to provide a basis for the development of gentiana safflower In-depth development and utilization to provide theoretical guidance

Method used

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  • Method for enriching and purifying mangiferin from gentiana rhodantha and detection method thereof
  • Method for enriching and purifying mangiferin from gentiana rhodantha and detection method thereof
  • Method for enriching and purifying mangiferin from gentiana rhodantha and detection method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Method for enriching and purifying mangiferin in Gentiana safflower:

[0059] 1. Preparation of safflower gentian dry extract and sample solution

[0060] Accurately weigh 320g of Gentiana safflower medicinal material powder, add 70% methanol according to the ratio of material to liquid 1:40 and reflux 3 times, 1h / time, combine the extracts, filter, and concentrate under reduced pressure to obtain 71.28g of extractum, Gentiana safflower 0.6 g of alcohol extraction extract, accurately weighed, placed in a conical flask, added 40 mL of water and ultrasonically dissolved for 30 min, prepared into a sample solution with a mass concentration of 0.0150 g / mL, and set aside.

[0061] 2. Kinetic adsorption of D101 resin to gentian mangiferin from safflower

[0062] 1) Put D101 macroporous resin into a glass chromatography column with distilled water for dynamic leaching to remove broken macroporous resin and impurities, then soak in 95% ethanol solution for 24 hours to fully ex...

Embodiment 2

[0067] Method for enriching and purifying mangiferin in Gentiana safflower:

[0068] 1. Preparation of safflower gentian dry extract and sample solution

[0069] Accurately weigh 320g of Gentiana safflower medicinal material powder, add 50% methanol according to the ratio of material to liquid 1:40 and reflux twice, 1h / time, combine the extracts, filter, and concentrate under reduced pressure to obtain 71.28g of extractum, Gentiana safflower 0.3 g of alcohol extraction extract, weighed accurately, placed in a conical flask, added 10 mL of water and ultrasonically dissolved for 20 min, prepared into a sample solution with a mass concentration of 0.0300 g / mL, and set aside.

[0070] 2. Kinetic adsorption of D101 resin to gentian mangiferin from safflower

[0071] 1) Put D101 macroporous resin into a glass chromatography column with distilled water for dynamic leaching to remove broken macroporous resin and impurities, then soak in 80% ethanol solution for 15 hours to fully expa...

Embodiment 3

[0076] Method for enriching and purifying mangiferin in Gentiana safflower:

[0077] 1. Preparation of safflower gentian dry extract and sample solution

[0078] Accurately weigh 320g of Gentiana safflower medicinal material powder, add 80% methanol according to the ratio of solid to liquid 1:40 and reflux 5 times, 1h / time, combine the extracts, filter, and concentrate under reduced pressure to obtain 71.28g of extractum, Gentiana safflower 1.2 g of alcohol extraction extract, accurately weighed, placed in a conical flask, added 160 mL of water and ultrasonically dissolved for 40 min, prepared into a sample solution with a mass concentration of 0.00750 g / mL, and set aside.

[0079] 2. Kinetic adsorption of D101 resin to gentian mangiferin from safflower

[0080] 1) Put D101 macroporous resin into a glass chromatography column with distilled water for dynamic leaching to remove broken macroporous resin and impurities, then soak in 95% ethanol solution for 30 hours to make it f...

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Abstract

The invention relates to a method for enriching and purifying mangiferin from gentiana rhodantha and a detection method thereof. Orthogonal test design and a macroporous resin adsorption purificationtechnology are adopted. Dynamic adsorption and desorption purification process parameters of mangiferin are optimized. An HPLC method is used as a detection means; according to the present invention,the influence of the D101 type macroporous adsorption resin on the mangiferin purification effect is discussed, and the method has characteristics of stability, feasibility and simple operation, is suitable for the enrichment and the purification of the mangiferin in the gentiana rhodantha, can provide the reference for the future biological activity research and the product development and utilization, and is suitable for the future industrial production of the gentiana rhodantha mangiferin.

Description

technical field [0001] The invention relates to the field of separation, purification and detection of active components of traditional Chinese medicines, in particular to a method for enriching and purifying mangiferin from Gentiana safflower and a detection method thereof. Background technique [0002] Safflower gentian (Gentiana rhodantha Franch.), also known as small herring gall grass, is a perennial herb of the family Gentianaceae Gentiana. It is a species recorded in the 2015 edition of "Chinese Pharmacopoeia" and the local standards of Guizhou, Sichuan and Hunan. [1-6] . The whole herb is used as medicine, with a bitter and cold taste. It has the effects of clearing heat and dehumidification, detoxifying and purging fire, and relieving cough. It is often used clinically to treat damp-heat jaundice, lung-heat cough, bronchitis, etc. [7] . Through literature review and analysis, safflower gentian is one of the plants with high mangiferin content, and has good anti-ox...

Claims

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Application Information

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IPC IPC(8): G01N30/08G01N30/06G01N30/02
CPCG01N30/08G01N30/06G01N30/02G01N2030/085
Inventor 郭江涛徐仕娟徐文芬孙庆文孙开芬张永萍
Owner 贵州中医药大学
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