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Platelet aggregation resistant aloe extract and application thereof in PRP preparation

A technology of anti-platelet aggregation and aloe vera extract, applied in the field of biomedicine, can solve the problems of plasma electrolyte disorder, reduction and osteoporosis, etc.

Pending Publication Date: 2020-08-14
湖南科诺康美生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The most commonly used anticoagulants are heparin sodium, sodium citrate, EDTA salt, oxalate, etc. However, these anticoagulants have more or less obvious disadvantages, such as the presence of sodium ions, which can easily lead to plasma electrolyte disturbance, and heparin can induce platelet Osteoporosis and osteoporosis, sodium citrate leads to Ca 2 + Ion imbalance, etc., especially when used in a large amount of human input, the side effects are more obvious, so the development and screening of new natural anticoagulants has important practical value

Method used

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  • Platelet aggregation resistant aloe extract and application thereof in PRP preparation
  • Platelet aggregation resistant aloe extract and application thereof in PRP preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the preparation of aloe extract

[0018] Weigh 100g of aloe vera powder, put it into a round bottom flask, add 1L of double distilled water, stir and mix well, put it in a water bath and heat it to reflux, heat at 100°C for 3h, filter it with cotton gauze, then centrifuge at 3000rpm for 3min, take the supernatant, Rotary steam under reduced pressure to 100mL, put in -80°C refrigerator overnight, and freeze-dry for 48 hours to obtain aloe vera extract.

experiment example 2

[0019] Experimental Example 2: The effect of aloe vera extract on anti-platelet aggregation induced by thrombin, ADP, collagen and arachidonic acid was detected by optical turbidimetry.

[0020] Platelet-rich plasma was prepared from rat blood by the common secondary centrifugation method, and 100 μL of platelet-rich plasma was taken, and the number of platelets was adjusted to 2.5×10 with PBS buffer solution (pH=7.4). 8 individual / mL. Take 400 μL of platelet resuspension, preheat at 37°C for 5 minutes, add aloe vera extract (final concentration is 0g / mL, 0.01g / L, 0.1g / L, 1g / L) and incubate at 37°C for 20min. Place the platelet plasma containing aloe vera extract in the test area of ​​the platelet aggregation instrument, after zero adjustment, add thrombin, ADP, collagen, arachidonic acid stimulator, stir at 1200rpm for 10min, use normal saline as a control, and measure platelets Aggregation rate, it was found that aloe extract can significantly reduce the effect of thrombin,...

example 3

[0021] Example 3: Aloe Vera Extract Combined with Sodium Citrate as Anticoagulant Complex

[0022] The preparation of the anticoagulant complex is that the mass ratio of sodium citrate and aloe extract is 1:1, and then a solution with a concentration of 4% sodium citrate is prepared. Add the anticoagulant solution dropwise into the anticoagulant tube, shake and hang it on the wall, then dry it, and add 20μL dropwise to 1mL blood for use. Collect the whole blood of the subject into a test tube containing anticoagulant, mix well and then centrifuge, take 100μL to calculate the number of platelets, distribute it to the centrifuge tube and centrifuge, centrifuge at 200g for 4min, separate into three layers, and use a pipette Extract the uppermost layer and most of the middle layer into a new centrifuge tube, centrifuge again, adjust the speed to 1500g, centrifuge for 20min, absorb 3 / 4 of the upper layer of plasma, and resuspend the precipitated platelets in the remaining 1 / 4 of th...

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PUM

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Abstract

The invention discloses a platelet aggregation resistant aloe extract and an application thereof in PRP preparation. The application comprises steps as follows: preparation of an aloe extract; ADP induced platelet aggregation resistance detection and PRP preparation and activation. The aloe extract has a remarkable inhibition function on ADP induced platelet aggregation, the aloe extract and sodium citrate are combined to be used as anticoagulants, prepared PRP has higher concentration and activity, and the PRP can be used to related clinical applications such as wound repair and the like after activation.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to an anti-platelet aggregation aloe polysaccharide and its application in the preparation of PRP. Background technique [0002] Platelet rich plasma (platelet rich plasma, PRP) comes from autologous whole blood or umbilical cord blood, which contains a higher concentration of platelets than whole blood / cord blood. It is widely used in tissue repair and regeneration, wound infection treatment and body function reconstruction. PRP participates in the hemostasis and immune response processes of platelet coagulation and fibrin production by releasing a large number of growth factors and cytokines required by the body. Therefore, the clinical therapeutic effect of PRP is mainly due to its secreted protein molecules and cytokines and other components. At present, there is no uniform standard to standardize the preparation of PRP, and the concentration and composition of platelets cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/46A61K47/12A61K35/16A61K36/896A61K36/886A61K31/194A61P9/00A61P31/04A61P7/04A61P29/00A61P17/02
CPCA61K47/46A61K47/12A61K35/16A61K36/896A61K36/886A61K31/194A61P9/00A61P31/04A61P7/04A61P29/00A61P17/02A61K2236/331A61K2300/00
Inventor 吴金芸雷登黄春兰
Owner 湖南科诺康美生命科技有限公司
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