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Preparation and use method of immobilized adsorbent for metal copper microorganisms in excrement

A microbial immobilization and adsorbent technology, applied in the field of animal husbandry, can solve problems such as unfavorable environmental protection and emission reduction, low cost, serious problems, etc., and achieve the effects of clear preparation and use principle, simple preparation, and enhanced adsorption capacity.

Pending Publication Date: 2020-08-14
YANGZHOU UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to propose a preparation of an adsorbent for immobilizing metal copper microorganisms in feces in view of the excessive copper present in the feces of livestock and poultry animals, which seriously pollutes soil, water sources, etc., and is not conducive to environmental protection and emission reduction. And the method of use, it is made by fixing and efficiently adsorbing microorganisms with sodium alginate gel, which has a good copper reduction effect, low cost and no secondary pollution, can reduce the excessive copper in livestock and poultry animal manure, and reduce the impact of high copper on the soil. , water pollution, etc., can obtain better economic benefits

Method used

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  • Preparation and use method of immobilized adsorbent for metal copper microorganisms in excrement
  • Preparation and use method of immobilized adsorbent for metal copper microorganisms in excrement
  • Preparation and use method of immobilized adsorbent for metal copper microorganisms in excrement

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preparation example Construction

[0029] A method for preparing an adsorbent for immobilizing metal copper microorganisms in feces, which is made by fixing and efficiently adsorbing microorganisms with sodium alginate gel, comprising the following steps:

[0030] The pre-screened high-efficiency copper-adsorbing microorganisms were selected as the dominant flora, namely Aspergillus oryzae, Aspergillus niger and Trichoderma. Take 5.0g of each of the above strains, add 50.0ml of warm water and 10.0g of nutrient agar, stir well, let stand for activation for 5 hours, and then set aside;

[0031] Select the raw materials for preparing the adsorbent, which are sodium alginate, calcium chloride, polyvinyl alcohol, distilled water, 0.5M HCl, and the required instruments and equipment are pH meter, syringe, glass rod and beaker;

[0032] Select the dominant bacterial group, follow the process parameters, and use the sodium alginate embedding method to make the metal copper microbial immobilization adsorbent. The proce...

Embodiment 1

[0045] Detection of the coating effect of the fecal metal copper microbial immobilization adsorbent

[0046] In order to explore the coating stability of microbial adsorption preparations and simulate their tolerance and integrity in acidic environment, alkaline environment, thermal environment and shaking environment, select 100 particles for each item and process them according to the method in Table (2). After the treatment is completed, count the number of intact particles, the number of particles with good hand-kneading texture, and good falling resilience for evaluation. The evaluation method is shown in Table (3), and the test results are shown in Table (2).

[0047] Table 2)

[0048]

[0049] table 3)

[0050]

[0051] From the test results in Table (2) and Table (3), it can be seen that after treatment with acid, alkali, heat and vibration, the adsorbent can still maintain high particle integrity, firm texture and drop resilience. It can be seen that the adsor...

Embodiment 2

[0052] Embodiment 2: Determination of the adsorption efficiency of the feces metal copper microbial immobilization adsorbent

[0053] A stool adsorption test was performed using this adsorbent. 5g of feces before and after adsorption were collected from the surface layer, the middle layer and the bottom layer respectively, and the contents of aflatoxin B1, ochratoxin and vomitoxin were determined to study whether the microbial adsorption would produce harmful mycotoxins. At the same time, adsorption parameters such as adsorption temperature, pH value, residual copper ion content in feces and adsorption rate were measured to determine its adsorption efficiency. The results are shown in the following table (4).

[0054] Table 4)

[0055]

[0056] Different lowercase letters on the data shoulder indicate significant differences among different groups (p0.05).

[0057] The results showed that there were no significant differences between the control group and the test group ...

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Abstract

The invention discloses a preparation and use method of an immobilized adsorbent for metal copper microorganisms in excrement, and belongs to the field of animal husbandry. The immobilized adsorbent is prepared by immobilizing and efficiently adsorbing microorganisms through sodium alginate gel, dominant strains are aspergillus oryzae, aspergillus niger and trichoderma, the safety of the strains is high, and the immobilized adsorbent has the advantages of accelerating excrement decomposition, killing pathogens and the like. The adsorbent is easy to prepare, low in price, high in gel strength and better in dryness resistance and acid and alkali resistance, the immobilized thallus cells can be desorbed after adsorbing copper and can be regenerated and recycled, the economic value of the adsorbent in use can be greatly improved, more binding sites on the surfaces of the cells are utilized after the thallus cells are immobilized, and the adsorption capacity is enhanced. The preparation anduse principles of the adsorbent are clear, the adsorption effect of the adsorbent is remarkable, and excessive copper in animal waste can be well reduced so that the harm to the environment is reduced, the average adsorption rate can reach 80%-90%, and the application prospect is wide.

Description

technical field [0001] The invention belongs to the field of animal husbandry and relates to a method for preparing and using an adsorbent, in particular to a method for preparing and using an adsorbent for immobilizing metallic copper microorganisms in feces. Background technique [0002] Copper (copper) is one of the trace elements necessary for the growth, development and maintenance of health of all living organisms, and it participates in many nutritional and metabolic processes in livestock and poultry. Studies have found that copper can reduce the expression level of somatostatin gene in young animals, thereby increasing the expression level of growth hormone releasing hormone, and can promote growth, especially for piglets. High copper (≥250mg / kg) can also improve Due to the feed conversion rate of young animals, farmers have added excessive copper (≥250mg / kg) to animal diets for a long time, causing a series of problems such as resource waste, animal copper poisonin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/28B01J20/34C02F11/02C02F101/20
CPCB01J20/24B01J20/28016B01J20/3475C02F11/02C02F2101/20B01J2220/4868
Inventor 甄永康王梦芝贡玉清李佩真顾亚兰张振斌于翔
Owner YANGZHOU UNIV
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