Construction method of recombinant baculovirus for expressing serum type 4 avian adenovirus fibroid protein F2
A technology of recombinant baculovirus and avian adenovirus, applied in the direction of virus, virus/phage, double-stranded DNA virus, etc., can solve the problem of lack of rapid and specific detection of FAdV-4, and achieve complete post-translational processing and modification ability, expression The effect of high level and high expression
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[0034] Below in conjunction with accompanying drawing and embodiment the present invention will be further described:
[0035] 1 material
[0036] 1.1 Strains, plasmids, cells
[0037] Serum type 4 avian adenovirus JH was isolated and identified in our laboratory. DH5α competent cells, DH10Bac competent cells, and Sf9 insect cells, pFastBacHTA plasmids are all preserved by our laboratory
[0038] 1.2 Main reagents
[0039] The monoclonal antibody against F2 protein was kept by the laboratory; Phanta Super-Fidelity DNA polymerase, Gene1Kb Super Marker, 2×Taq Master Mix and ExnaseTMII ligase were purchased from Nanjing Novozyme Company; the plasmid mini-prep kit was purchased from AxyPrep Company ; Gel recovery DNA kit was purchased from QIAGEN; LipofectamineTM3000 transfection reagent and FBS fetal bovine serum were purchased from Gibco; goat anti-mouse FITC-labeled IgG was purchased from SIGMA; Western-blot exposure solution was purchased from Nanjing Feerte Biotechnology ...
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