Method for quantitatively detecting concentration of bismuth in serum by inductively coupled plasma mass spectrometry

Abstract

The invention discloses a method for quantitatively detecting the concentration of bismuth in serum by inductively coupled plasma mass spectrometry. The method comprises the steps of firstly, nitrifying a beagle serum sample by adopting nitric acid, and detecting the content of bismuth in beagle serum by taking rhodium as an internal standard and adopting an inductively coupled plasma mass spectrometry (ICP-MS) method. The mass spectrometry detection parameters are as follows: the radio frequency power is 1550W; the pump speed is 40r.min<-1>; the temperature of an atomizing chamber is 2.7 DEGC; the sampling depth is 5mm; the cooling air flow velocity is 14L.Min<-1>; the auxiliary air flow velocity is 0.8L.min<-1>; and the atomized air flow velocity is 1.1L.min<-1>. According to the method, the linear relation of folic acid at 0.2-50ng / ml is good. The recovery rate of the method ranges from 101.1% to 103.4%; the accuracy in batches and the accuracy between batches are 91.9%-101.4%, andthe precision in batches and the precision between batches are 3.2%-10.5%. The method is low in limit of quantitation, short in analysis time and good in reproducibility, can meet the detection requirements of the concentration of bismuth in beagle serum, and can be used for pharmacokinetic research of colloidal bismuth citrate capsules.

Description

technical field [0001] The invention belongs to the field of biological analysis, in particular to a method for quantitatively detecting bismuth concentration in serum by inductively coupled plasma mass spectrometry. Background technique [0002] Colloidal bismuth citrate capsules are suitable for treating peptic ulcers, especially Helicobacter pylori-related ulcers; they can also be used for chronic colitis, diarrhea caused by ulcerative colitis, and chronic superficial and atrophic gastritis. However, bismuth is a heavy metal, long-term use or overdose will lead to accumulation in the body and cause nephrotoxicity and other toxic and side effects. Therefore, dynamic monitoring of bismuth concentration in the body after administration is very important for rationally explaining and explaining its curative effect and reducing toxic and side effects. Traditional detection methods include electrochemical methods, atomic absorption spectrometry, and atomic fluorescence spectro...

AI Technical Summary

Problems solved by technology

Traditional detection methods include electrochemical methods, atomic absorption spectrometry, and atomic fluorescence spectrometry, but the above methods have limited sensitivity and are difficult to apply to the detection of drug concentrations in serum in vivo and pharmacokinetic studies

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