A kind of pharmaceutical composition for treating lung cancer and its preparation method
A composition and drug technology, applied in the field of medicine, to achieve the effects of enhancing curative effect, reducing side effects, and improving drug resistance
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Embodiment 1
[0021] Example 1. Neogambogic acid combined with crocin inhibits the proliferation of A549 cell lines
[0022] 1. Experimental materials
[0023] (1) Experimental cells:
[0024] Human lung cancer cell line (A549) was purchased from Shanghai Institute of Cell Biology, Chinese Academy of Sciences.
[0025] (2) Pharmaceutical reagents:
[0026] New gambogic acid was purchased from Shanghai Yansheng Industrial Co., Ltd.;
[0027] Crocin was purchased from Sigma, USA.
[0028] 2. Experimental method
[0029] (1) Culture and treatment of human lung cancer cells (A549)
[0030] Human lung cancer cells (A549) were cultured in DMEM medium containing 10% fetal bovine serum and 1% double antibody (100U / ml penicillin, 100mg / L streptomycin), 37°C, 5% CO 2 Subculture in an incubator. The cells were subcultured twice a week, and the adherent cells were digested with 0.25% trypsin. The cells used in the experiment were all in the logarithmic growth phase.
[0031] (2) MTT method to d...
Embodiment 2
[0041] Example 2. Effect of neogambogic acid combined with crocin on inhibiting the growth of A549 cell transplanted tumor in nude mice
[0042] 1. Inoculation of A549 cell transplanted tumors in nude mice: BALB / c nude mice, 5-6 weeks old, male, weighing about 20 g, were bred. Subcutaneous inoculation and transplantation of cultured cells was adopted. Take A549 cells in the logarithmic growth phase, rinse the culture bottle twice with PBS, add 0.25% trypsin to digest, centrifuge and discard the supernatant, add 10ml of DMEM culture solution without fetal bovine serum, pipette to mix, discard the supernatant, repeat 1 time, then add serum-free DMEM medium, resuspend the cells, and adjust the cells to about 3×10 7 200 μl per mouse was inoculated subcutaneously under the armpit of the right forelimb of nude mice.
[0043] 2. Inhibitory effect of drugs on lung cancer xenografts in nude mice: the tumor grew 2 weeks after inoculation, and the tumor grew to 100-150mm 3 After that,...
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