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Hyaluronic acid hydrogel and preparation method and application thereof

A technology of hyaluronic acid and hydrogel, which is used in pharmaceutical formulations, medical preparations with non-active ingredients, medical science, etc., can solve the problem of limited application, weak mechanical properties of double-bonded hyaluronic acid hydrogel, and permanent rupture. and other problems, to achieve the effect of comprehensive performance improvement, mechanical strength improvement, and swelling performance improvement.

Pending Publication Date: 2020-08-28
GUANGDONG PROV MEDICAL INSTR INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional covalent chemically cross-linked double-bond hyaluronic acid hydrogels have weak mechanical properties and are prone to permanent rupture under external force, which severely limits their application in tissue repair.

Method used

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  • Hyaluronic acid hydrogel and preparation method and application thereof
  • Hyaluronic acid hydrogel and preparation method and application thereof
  • Hyaluronic acid hydrogel and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Preparation of double bond modified hyaluronic acid

[0038] Stir and dissolve 1 g of hyaluronic acid in 90 mL of mixed solvent (DMF 30 mL, water 60 mL). After fully dissolving, cool down to 4°C on ice, and adjust the pH to between 8 and 9 with 10 mol / L sodium hydroxide solution. Slowly add 3.7 mL of methacrylic anhydride dropwise, and stir overnight in an ice-water bath. The reaction needs to stabilize the pH value between 8-9. Pour the reacted solution into 500mL of absolute ethanol, centrifuge after the precipitation of MA-HA (methacrylic anhydride modified hyaluronic acid) is complete, the precipitated product is redissolved, dialyzed through a dialysis bag with a molecular weight of 14000, and frozen after 5 days of dialysis dry to obtain methacrylic anhydride-modified hyaluronic acid.

[0039] 2. Preparation of hyaluronic acid hydrogel enhanced by small molecule cross-linking agent

[0040] 2% (w / v, 20mg / mL) double bond modified hyaluronic acid aqueous solu...

Embodiment 2

[0043] 1. Preparation of double bond modified hyaluronic acid

[0044] The preparation method of double bond modified hyaluronic acid in this example is the same as that in Example 1.

[0045] 2. Preparation of hyaluronic acid hydrogel enhanced by small molecule cross-linking agent

[0046] 2% (w / v, 20mg / mL) double bond modified hyaluronic acid aqueous solution, 0.1% N,N'-ethylenebisacrylamide, 0.08% (w / v, 0.8mg / mL) LAP (Phenyl-2,4,6-trimethylbenzoyllithium phosphonate) photoinitiator, vortexed to make a homogeneous solution. 365nm ultraviolet light cross-linked for 5s (seconds) to form a gel. Place at room temperature for 12h.

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Abstract

The invention discloses hyaluronic acid hydrogel and a preparation method and application thereof. The hyaluronic acid hydrogel is formed by double-bond cross-linking of double-bond modified hyaluronic acid and a small molecular cross-linking agent. The preparation method of the hyaluronic acid hydrogel comprises the following step: carrying out photo-initiation crosslinking on the double-bond modified hyaluronic acid, the small molecular crosslinking agent and a photoinitiator to obtain the hyaluronic acid hydrogel. According to the micromolecular cross-linking agent enhanced hyaluronic acidhydrogel, the mechanical strength is remarkably improved, the swelling performance is greatly improved, the comprehensive performance is remarkably improved, and the application prospect is wide.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a hyaluronic acid hydrogel and its preparation method and application. Background technique [0002] Hydrogel is a network polymer that swells in water and retains water in the polymer voids, and has a wide range of applications in the field of biomaterials. Hyaluronic acid (HA) is a polysaccharide widely used in biomedicine. It is rich in anions and has the ability to resist protein adhesion. However, traditional covalent chemically crosslinked double-bond hyaluronic acid hydrogels have weak mechanical properties and are prone to permanent rupture under external force, which severely limits their application in tissue repair. How to solve the problems existing in the existing traditional chemically cross-linked hydrogels and develop a new hyaluronic acid hydrogel with enhanced mechanical properties has become a hot topic of concern for researchers in this field. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F251/00C08F222/38C08F2/48A61K47/36A61L27/52A61L27/50A61L27/20
CPCC08F251/00C08F2/48A61K47/36A61L27/52A61L27/50A61L27/20C08F222/385C08L5/08
Inventor 于珊纪禹行耿志杰刘群峰
Owner GUANGDONG PROV MEDICAL INSTR INST