Cross-linking modification method of antibacterial gelatin

A technology of cross-linking modification and gelatin, which is applied in medical science, surgery, etc., can solve the problems of no antibacterial properties, and achieve the effects of improving safety, good biocompatibility, and superior mechanical properties

Inactive Publication Date: 2020-08-28
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Usually cross-linked raw gelatin has no antibacterial properties, and most of the reported cross-linked gelatins with anti

Method used

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  • Cross-linking modification method of antibacterial gelatin
  • Cross-linking modification method of antibacterial gelatin
  • Cross-linking modification method of antibacterial gelatin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] A cross-linking modification method of antibacterial gelatin, comprising steps as follows:

[0058] (1) Dissolve 2.5g of gelatin in 22.5g of phosphate buffer solution with a pH of 10 to prepare a gelatin solution with a mass fraction of 10wt%. After swelling at room temperature for one hour, heat and magnetically stir in a water bath at 50°C for 1 hour to dissolve.

[0059] (2) add 0.486g N in the 10wt% gelatin solution that prepares, N dimethyl glycidyl octadecyl ammonium chloride, make the mol ratio of epoxy group in gelatin primary amino group and quaternary ammonium salt be 1 : 0.5, keep the temperature at 50°C for 5h under magnetic stirring.

[0060] (3) After the quaternization reaction is completed, add 1 mL of a cross-linking agent tannic acid aqueous solution of 200 mg / mL configured in the reaction system, so that the mass ratio of the cross-linking agent to antibacterial gelatin is 0.07:1, and the concentration of the cross-linking agent to 0.76%, continue to...

Embodiment 2

[0066] A cross-linking modification method of antibacterial gelatin, comprising steps as follows:

[0067] (1) Dissolve 2.5g of gelatin in 22.5g of buffer solution with a pH of 9 to prepare a gelatin solution with a mass fraction of 10wt%. After swelling at room temperature for one hour, heat in a water bath at 50°C with magnetic stirring to dissolve for 1 hour.

[0068] (2) add 0.972gN in the 10wt% gelatin solution that prepares, N dimethylglycidyl octadecyl ammonium chloride, make the mol ratio of epoxy group in gelatin primary amino group and quaternary ammonium salt be 1: 1. Keep the temperature at 45°C for 5 hours under magnetic stirring.

[0069] (3) After the quaternization reaction is completed, add 1 mL of a cross-linking agent tannic acid aqueous solution of 200 mg / mL to the reaction system, so that the mass ratio of the cross-linking agent to antibacterial gelatin is 0.06:1, so that the cross-linking agent The concentration was 0.74%, and the temperature was kept a...

Embodiment 3

[0073] A cross-linking modification method of antibacterial gelatin, comprising steps as follows:

[0074] (1) Dissolve 2.5g of gelatin in 22.5g of phosphate buffer solution with a pH of 11 to prepare a gelatin solution with a mass fraction of 10wt%. After swelling at room temperature for one hour, heat and magnetically stir in a water bath at 50°C for 1 hour to dissolve.

[0075] (2) add 1.458gN in the 10wt% gelatin solution that prepares, N dimethylglycidyl octadecyl ammonium chloride, make the mol ratio of epoxy group in gelatin primary amino group and quaternary ammonium salt be 1: 1.5, keep the temperature at 55°C for 5h under magnetic stirring.

[0076] (3) After the quaternization reaction is completed, add 1 mL of a cross-linking agent tannic acid aqueous solution of 200 mg / mL to the reaction system, so that the mass ratio of the cross-linking agent to antibacterial gelatin is 0.05:1, so that the cross-linking agent The concentration was 0.73%, and the temperature was...

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Abstract

The invention relates to a cross-linking modification method of antibacterial gelatin. The method comprises the following steps: 1, a natural biological material is used as a cross-linking agent to besubjected to a cross-linking reaction with antibacterial gelatin, wherein the pH of a solution in the cross-linking reaction is 9-11, the concentration of the cross-linking agent is 0.5-0.8 wt%, themass ratio of the cross-linking agent to the antibacterial gelatin is (0.03-0.08):1, the temperature of the cross-linking reaction is 42-58 DEG C, and the time of the cross-linking reaction is 2-10 h.The natural biological cross-linking agent is selected for cross-linking modification and the mechanical property of the antibacterial gelatin is improved; meanwhile, no toxic or side effect is generated on the human body, and the use safety of the gelatin material is improved. In addition, crosslinking modification conditions are optimized, so that the crosslinked gelatin with good biocompatibility and excellent mechanical properties is obtained, and the application of the crosslinked gelatin in medical antibacterial materials is facilitated.

Description

technical field [0001] The invention relates to a cross-linking modification method of antibacterial gelatin, which belongs to the technical field of antibacterial materials. Background technique [0002] Gelatin is a polypeptide compound formed by the hydrolysis of collagen in connective tissues such as animal skin and bones. It is valued for its low immune activity, biodegradability, and ability to promote cell proliferation and division. Gelatin is a natural renewable resource with a wide range of sources and low price. It has good film-forming properties, biocompatibility and biodegradability, and its degradation products are easily absorbed without inflammatory reactions. It is an excellent biomedical material. Pure gelatin is brittle after drying, weak in formability, low in ductility, and susceptible to bacterial erosion and deterioration in humid environments. Therefore, different modification methods must be used to improve the mechanical and antibacterial propertie...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08L89/00C08K5/1545C08H1/00A61L31/16A61L31/14A61L31/04
CPCA61L31/045A61L31/14A61L31/16A61L2300/252A61L2300/404C08H1/00C08J3/24C08J2389/00C08K5/1545
Inventor 李俊英于宁马烽耿海滨杨鹏飞孙海霖
Owner QILU UNIV OF TECH
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