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Octenyl succinic anhydride modified gelatin as well as preparation method and application thereof

A technology of octenyl succinic anhydride and gelatin, which is applied in the direction of application, medical preparations of non-active ingredients, dairy products, etc., to achieve broad application prospects, improve stability, and improve the effect of emulsion stability

Active Publication Date: 2020-09-04
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, proteins modified by OSA have not been used as emulsifiers to stabilize emulsions

Method used

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  • Octenyl succinic anhydride modified gelatin as well as preparation method and application thereof
  • Octenyl succinic anhydride modified gelatin as well as preparation method and application thereof
  • Octenyl succinic anhydride modified gelatin as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The preparation of embodiment 1 OSA-BBG and OSA-CFG

[0053] (1) Determination of amino acid content

[0054] Determination of the amino acid content of bovine bone gelatin (BBG) and cold water fish skin gelatin (CFG), weigh 1.0g of gelatin powder (BBG and CFG) into a 30mL hydrolysis tube, immediately add 10mL HCl (6mol / L) and 0.15mL phenol . The samples were refrigerated at 4°C for 5 min. Then, the sample was evacuated for 10 min and hydrolyzed at 110 °C for 22 h. The hydrolyzate was filtered with filter paper, collected in a 50mL volumetric flask and made up to 50mL with ultrapure water. The vacuum-dried sample was dissolved in a mixture of 20 mL of sodium citrate buffer (66.6 mM, pH 2.2) and ultrapure water at a volume ratio of 1:19. Samples and commercial mixed amino acid standards (sigma-Aldrich, USA) were analyzed using an L-8800 automatic amino acid analyzer. Each group of samples was measured 3 times in parallel, and the results are shown in Table 1.

[00...

Embodiment 2

[0077] Embodiment 2 prepares OSA modified gelatin stable carrier fish oil emulsion

[0078] The unmodified and OSA-modified gelatin solutions (1.0%, w / v) with different degrees of succinylation prepared in Example 1 were obtained by dissolving, and the pH of the solution was adjusted to 9.0. Add fish oil to the gelatin solution at a volume ratio of 1:2. then use The homogenizer performs homogenization, and the homogenization speed and time are 11500 rpm and 1 min, respectively. The resulting emulsion was stored at 4°C. Emulsions were observed with a digital camera, optical microscope and confocal laser scanning microscope (CLSM).

[0079] (1) Detection method

[0080] 1. Optical microscope observation

[0081] 3 μL of the liquid emulsion sample was dropped onto a glass slide, covered with a cover glass, and observed with an inverted optical microscope (MS600F, Shanghai Mingzi Precision Instrument Co., Ltd., China). About 3 mg of the emulsion gel sample was cut and place...

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Abstract

The invention relates to the field of medical and food additives, and discloses octenyl succinic anhydride modified gelatin. The preparation method comprises the following steps: (1) regulating the pHvalue of a gelatin solution to 8-9, and adding octenyl succinic anhydride while stirring to react for 2-6 hours, wherein the mass ratio of octenyl succinic anhydride to gelatin is (0.02-0.25): 1; and(2) adjusting the pH value of the solution to 6-7, and removing the part of MW8000-14000Da by dialysis. Octenyl succinic anhydride is mixed with the gelatin solution. The modified gelatin can be applied to preparation of an emulsifier, and when the emulsifier is applied to preparation of an emulsion, the emulsion index can be remarkably reduced, and the droplet stability can be improved. The modified gelatin has a wide application prospect in the fields of foods, medicines and the like.

Description

technical field [0001] The invention relates to the field of medical food additives, specifically emulsifiers, especially gelatin modified with octenyl succinic anhydride as emulsifiers. Background technique [0002] Fish oil has many important physiological and health functions for the human body, and fish oil is a good carrier of fat-soluble substances. However, fish oil has a fishy smell, poor water solubility and is easily oxidized by environmental factors (pH, light, heat, etc.). Fish oil-loaded emulsions have great potential to reduce the restrictions on the application of fish oil, and have attracted great attention in the past 20 years. [0003] Mammalian and aquatic gelatins have been used as emulsifiers for o / w emulsions due to their excellent edibility, biocompatibility, and degradability. Kraft gelatin and fish gelatin have been used as emulsifiers to stabilize sunflower and corn oils. Due to mammalian diseases and religious factors, the wide application of ma...

Claims

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Application Information

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IPC IPC(8): C08H1/00A61K9/10A61K47/42A61K47/22A23L2/52A23C9/152
CPCC08H1/00A61K9/10A61K47/42A61K47/22A23L2/52A23C9/1526A23V2002/00A23V2200/222A23V2250/5432
Inventor 钟建张婷丁梦真陶丽娜王锡昌
Owner SHANGHAI OCEAN UNIV
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