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Method for improving toxicity resistance of microalgae in wastewater treatment process through brassinolide

Brassinolide, a technology for treating wastewater, applied in the directions of microorganism-based methods, sustainable biological treatment, chemical instruments and methods, etc., can solve the problem of decreased yield of additional products of microalgae, pollutant toxicity stress yield, energy consumption and the increase of training costs, etc., to achieve the effect of low cost, environmental friendliness and convenient operation.

Active Publication Date: 2020-09-11
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the current methods have improved the lipid productivity of microalgae cells to a certain extent, the energy consumption and culture costs have increased significantly, and it is not practical after economic analysis
[0004] Moreover, the above method is only applicable to the conventional culture process of microalgae
In the process of using microalgae to treat wastewater, due to the toxic stress of pollutants in wastewater, the growth of microalgae is seriously affected, and the yield of additional products of microalgae decreases.
How to solve the problem of yield decline due to pollutant toxicity stress in the process of microalgae treatment of wastewater and resource transformation, and provide a method for improving toxicity resistance in the process of microalgae treatment of wastewater has not been reported so far

Method used

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  • Method for improving toxicity resistance of microalgae in wastewater treatment process through brassinolide
  • Method for improving toxicity resistance of microalgae in wastewater treatment process through brassinolide
  • Method for improving toxicity resistance of microalgae in wastewater treatment process through brassinolide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Microalgae enrichment

[0062] Add 3LBG11 medium into a 5L photoreactor, and inoculate the cells of Chlorella, Scenedesmus, Chlamydomonas and Spirulina into the medium at a volume ratio of 15%, respectively, at 20°C, with a light-to-dark ratio of 16:8 and Cultivate at a rotational speed of 150r / min until the concentration of microalgae is 3×10 7 individual / mL.

[0063] Among them, the formula of BG11 medium is: 1.5g / LNaNO 3 , 2.86g / L H 3 BO 4 , 0.04g / L K 2 HPO 4 ·3H 2 O, 1.86g / LMnCl 2 , 0.075g / L MgSO 4 ·7H 2 O, 0.08g / L CuSO 4 , 0.036g / L CaCl 2 .2H 2 O, 0.22g / LZnSO 4 , 0.006g / L Lemon, 0.39g / LNa 2 MoO 4 , 0.006g / L ferric ammonium citrate, 0.05g / L Co(NO 3 ) 2 ·6H 2 O, 0.02g / LNaCO 3 , 0.001g / L EDTANa 2 .

Embodiment 2

[0065] Improvement of microalgae biomass by brassinolide in wastewater culture

[0066] 1) Dilute the pharmaceutical wastewater, chemical wastewater and beer wastewater 10 times with distilled water, respectively, and then sterilize them, and measure the indicators of the wastewater to be treated;

[0067] 2) Add three kinds of sterilized waste water and 500 μg / L brassinosteroid respectively in the photoreactor, and use the stirring rod provided in the reactor to stir clockwise for 15 s and counterclockwise for 15 s in turn, Stir for 3 minutes until there is no obvious aggregation of microalgae clusters;

[0068] 3) After mixing, add the concentration of 3×10 7 Chlorella, Scenedesmus, Chlamydomonas and Spirulina in individual / mL with a volume fraction of 15%;

[0069] 4) Adjust the temperature to 20° C., use LED light to illuminate, the light-to-dark ratio is 16 / 8, and the rotation speed is 150 r / min to measure the biomass of microalgae after culturing for 72 hours.

[0070...

Embodiment 3

[0078] Improvement of microalgae biomass by brassinolide in wastewater culture

[0079] Concrete method is the same as embodiment 2, and in step 3), adding volume fraction is 5% Chlorella, Scenedesmus, Chlamydomonas and Spirulina respectively, and the results are shown in Table 3:

[0080] Brassinolide (500 μg / L) in different wastewaters in table 3 promotes the biomass of microalgae (5% microalgae)

[0081]

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Abstract

The invention relates to the technical field of wastewater treatment, in particular to a method for improving the toxicity resistance of microalgae in the wastewater treatment process through brassinolide. According to the invention, a trace amount of brassinolide is added, so that the toxicity resistance of microalgae is improved, the metabolic activity of microalgae is optimized, the yield of microalgae biomass in a wastewater biological treatment and microalgae biomass energy coupling technology is increased, and the synthesis of high-value additional products is directionally adjusted.

Description

technical field [0001] The invention belongs to the technical field of waste water treatment, and in particular relates to a method for improving the toxicity resistance of microalgae in the process of treating waste water through brassinolide. Background technique [0002] As the raw material of the third-generation biofuel, microalgae has high photon conversion efficiency, fast growth, small footprint and simple cultivation method compared with traditional carbohydrates. Because microalgae have a good fatty acid composition, its oil composition is similar to that of oil-rich plants. The carbon chain length of fatty acids is mainly C16 and C18, and most of them are neutral lipids, which are usually stored in the form of triacylglycerol (TAG). It is the main source of biodiesel. Microalgae accumulate lipids through physiological activities, and the lipid content can be as high as 30%-80% of the dry cell weight. Not only that, microalgae can also produce a variety of high-v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/32C12N1/12C12R1/89
CPCC02F3/325C12N1/12Y02W10/10
Inventor 周丹丹赵振豪付亮崔晓春刘洋
Owner NORTHEAST NORMAL UNIVERSITY
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