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Helicobacter pylori magnetic particle chemiluminiscence detection kit and preparation method thereof

A technology for detection of Helicobacter pylori and chemiluminescence, which is applied in chemiluminescence/bioluminescence, analysis through chemical reactions of materials, and measurement devices, can solve the problems of long test time, poor accuracy, and poor stability, and achieve The effect of fast test speed, improved sensitivity and stable reagent performance

Inactive Publication Date: 2020-09-11
DIRUI MEDICAL TECH CO LTD
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  • Abstract
  • Description
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Problems solved by technology

[0005] The present invention aims to solve the technical problems of long testing time, poor repeatability, poor stability, poor sensitivity, and poor accuracy existing in the existing Helicobacter pylori detection method, and provides a high-efficiency, high-stability Helicobacter pylori magnetic particle chemiluminescence detection Kit and preparation method thereof

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  • Helicobacter pylori magnetic particle chemiluminiscence detection kit and preparation method thereof
  • Helicobacter pylori magnetic particle chemiluminiscence detection kit and preparation method thereof
  • Helicobacter pylori magnetic particle chemiluminiscence detection kit and preparation method thereof

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preparation example Construction

[0048] The preparation method of the Helicobacter pylori magnetic particle chemiluminescence detection kit of the present invention comprises the following steps:

[0049] Step 1, R1 reagent preparation:

[0050] Take the streptavidin magnetic particle solution and add it to the TBST solution, mix it well, place it on the magnetic separator until the supernatant is free of turbidity, discard the supernatant, keep the magnetic particle, wash it three times with buffer Ⅰ, after washing , and then use buffer I to prepare the solid-phase reagent of streptavidin magnetic particle solution, which is the R1 reagent;

[0051] Step 2, R2 reagent preparation:

[0052] Put the Helicobacter pylori antibody into the centrifuge tube, make sure that the antibody is at the bottom of the centrifuge tube, add carbonic acid buffer solution, mix well, add the chemiluminescence substance solution after mixing, and centrifuge at room temperature in a centrifuge; cover the centrifuge tube with a pa...

Embodiment 1

[0067] Preparation of Helicobacter pylori magnetic particle chemiluminescent detection kit:

[0068] Preparation of R1 reagent: Take 0.5 mL (50 mg) of streptavidin magnetic particle (particle size 1 μm) solution with a concentration of 100 mg / mL and add it to the TBST solution, mix well, and place it on a magnetic separator until the supernatant is free. If it is turbid, discard the supernatant, keep the magnetic particles, and wash them three times with buffer I. After washing, use buffer I to make a solid-phase reagent with a magnetic particle concentration of 0.05%, which is the R1 reagent, and store at 2-8°C;

[0069] Preparation of R2 reagent: put 500 μg of Helicobacter pylori antibody into a centrifuge tube, make sure that the antibody is at the bottom of the centrifuge tube (centrifuge at room temperature for 20 seconds), then add carbonic acid buffer solution, mix well, add 5 μL of 2mg / mL acridine after mixing Centrifuge the DMF solution of the ester in a centrifuge at...

Embodiment 2

[0074] Preparation of R1 reagent: Take 0.5 mL (50 mg) of a solution of streptavidin magnetic particles (particle size 3 μm) with a concentration of 100 mg / mL and add it to the TBST solution, mix well, and place it on a magnetic separator until the supernatant is free If it is turbid, discard the supernatant, keep the magnetic particles, and wash them three times with buffer I. After washing, use buffer I to make a solid-phase reagent with a magnetic particle concentration of 1%, which is the R1 reagent, and store at 2-8°C;

[0075] Preparation of R2 reagent: put 500 μg of Helicobacter pylori antibody into a centrifuge tube, make sure that the antibody is at the bottom of the centrifuge tube (centrifuge at room temperature for 20 seconds), then add carbonic acid buffer solution, mix well, add 5 μL of 2mg / mL acridine after mixing Centrifuge the DMF solution of the ester in a centrifuge at room temperature for 0.5 min; seal the centrifuge tube with a parafilm and put it into a lig...

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Abstract

The invention relates to a helicobacter pylori magnetic particle chemiluminiscence detection kit and a preparation method thereof, and relates to the technical field of chemiluminiscence in-vitro diagnosis. The technical problems that an existing helicobacter pylori detection method is long in test time, poor in repeatability, poor in stability, poor in sensitivity and poor in accuracy are solved.The kit comprises a streptavidin magnetic particle solution, a chemiluminescent substance labeled helicobacter pylori antibody solution, a biotin labeled helicobacter pylori antibody solution, a calibration product and a quality control product. The invention also provides a preparation method of the helicobacter pylori magnetic particle chemiluminiscence detection kit. The kit provided by the invention directly detects the concentration of helicobacter pylori in serum by adopting a homogeneous magnetic particle luminescence technology and a sandwich method, and has the advantages of high test speed, good test result repeatability, stable reagent performance, high sensitivity, accurate test result and the like.

Description

technical field [0001] The invention relates to the technical field of chemiluminescence in vitro diagnosis, in particular to a Helicobacter pylori magnetic particle chemiluminescence detection kit and a preparation method thereof. Background technique [0002] Helicobacrer pylori (Helicobacrer pylori, HP) is a Gram-negative microaerophilic bacterium that parasitizes the stomach and duodenum. Its infection is very common, and more than 50% of the global natural population is infected. Almost all Helicobacter pylori-infected patients have chronic active inflammatory reactions histologically. The latest expert consensus defines HP gastritis as "an infectious (infectious) disease", but more than 70% of them have no symptoms. Symptoms, others can lead to chronic gastritis, peptic ulcer, etc. Studies have shown that Helicobacter pylori is the main pathogenic factor of gastritis and peptic ulcer, and is closely related to the occurrence of functional dyspepsia, gastric mucosa-ass...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543G01N21/76
CPCG01N21/76G01N33/54326G01N33/56922G01N2469/10
Inventor 高阳唐仁陶于猛高威孙成艳何浩会
Owner DIRUI MEDICAL TECH CO LTD
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