Thrombolytic efficacy evaluation method based on two-photon microscope technology

Abstract

The invention discloses a thrombolytic efficacy evaluation method based on a two-photon microscope technology. The thrombolytic efficacy evaluation method comprises the following steps: (1) establishing a cerebral thrombosis animal model of a living animal in a waking state, wherein the cerebral thrombosis animal model comprises an arterial thrombosis model and a venous thrombosis model; (2) carrying out fluorescence labeling on a thrombolytic drug by adopting fluorescein isothiocyanate to obtain a fluorescein isothiocyanate labeled thrombolytic drug; (3) injecting the fluorescein isothiocyanate labeled thrombolytic drug into the caudal vein of the cerebral thrombosis animal model; (4) detecting hemodynamics and vascular morphological changes after thrombolytic drug treatment, and observing changes of blood oxygen pressure after thrombolytic drug treatment; (5) observing the change of the cerebral infarction area after thrombolytic drug treatment, and judging the cerebral area range affected by thrombus; and (6) judging the efficacy of the thrombolytic drug according to the result. The method has the characteristic that the efficacy of the thrombolytic drug can be directly evaluated.

Description

technical field [0001] The invention relates to a method for evaluating thrombolytic efficacy, in particular to a method for evaluating thrombolytic efficacy based on two-photon microscope technology. Background technique [0002] As my country's population aging process continues to accelerate, the incidence of stroke is increasing year by year. In 2018, the number of stroke patients in my country has reached 12.42 million, of which stroke caused by cerebral thrombosis accounts for 50% of stroke patients, which has become a threat to the health of middle-aged and elderly people in my country. It has a high disability rate, causes great suffering to patients, and also causes great burden to family members and society. One of the main components of thrombus is fibrin. Thrombolytic drugs can directly or indirectly activate plasminogen into plasmin, which can degrade fibrin (ogen), promote the lysis of thrombus and achieve the purpose of opening blood vessels. Thrombolytic ther...

AI Technical Summary

Problems solved by technology

The drug efficacy results reflected by in vivo tests are highly reliable and are relatively close to the actual clinical application. Therefore, the results of in vivo tests are often the main indicators for drug efficacy evaluation, but the efficacy of drugs can only be judged indirectly through changes in animal behavior, and cannot be observed in real time. After drug treatment, the dynamic changes of thrombus in the brain, the changes of hemodynamics and the changes of blood vessel morphology

Therefore, the existing technology has the problem of being unable to directly evaluate the efficacy of thrombolytic drugs

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