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Method for preparing high-efficiency lactic acid bacteria culture medium by liquid-state fermentation of shredded edible fungi

A technology of liquid fermentation and lactic acid bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of complex components of MRS medium and high cost of cultivating lactic acid bacteria, and achieve simple production, low cost, and reduce the number of deaths Effect

Inactive Publication Date: 2020-09-15
HUNAN UNIV OF SCI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects of complex MRS medium components and high cost of cultivating lactic acid bacteria in the prior art, and provide a method for preparing a high-efficiency liquid culture medium for lactic acid bacteria from edible mycelium

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  • Method for preparing high-efficiency lactic acid bacteria culture medium by liquid-state fermentation of shredded edible fungi
  • Method for preparing high-efficiency lactic acid bacteria culture medium by liquid-state fermentation of shredded edible fungi

Examples

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Embodiment 1

[0023] A method for preparing a high-efficiency lactic acid bacteria medium by liquid fermenting edible mycelia, comprising the following steps:

[0024] S1. Weigh an appropriate amount of liquid fermented shiitake mushroom mycelium 225g, add water to high-speed grinding in a wall breaker to make a mycelium slurry, add an appropriate amount of water to the mycelium slurry, boil for 15-25 minutes, and then use two layers of medical Filter through gauze, add 5 g of sodium acetate and 0.6 mg of riboflavin, and dilute the final volume of the filtrate to 1000 mL by adding water;

[0025] S2. Adjust the pH value to between 6.2-6.4 to obtain the filtrate culture medium, divide it into 50mL Erlenmeyer flasks, and sterilize at 121°C for 20min;

[0026] S3. Inoculate the seed liquid of Lactobacillus plantarum LP-G18 into the above-mentioned filtrate medium, and culture it at 37°C.

[0027] The mycelium of Pleurotus eryngii is rich in protein and free amino acids, especially aspartic ac...

Embodiment 2

[0029] A method for preparing a high-efficiency lactic acid bacteria medium by liquid fermenting edible mycelia, comprising the following steps:

[0030] S1. Weigh an appropriate amount of liquid fermented shiitake mushroom mycelium 200g, add water to high-speed grinding to make mycelium slurry, add appropriate amount of water to the mycelium slurry, boil for 15-25 minutes, then filter with two layers of medical gauze, add lemon Ammonium acid 1.5g / L, MgSO 4· 7H 2 O 0.3 mg, the final volume of the filtrate was constant to 1000 mL by supplementing water;

[0031] S2. Adjust the pH value to between 6.8 to obtain the filtrate culture medium, divide it into 50mL Erlenmeyer flasks, and sterilize at 121°C for 20min;

[0032] S3. Inoculate the seed liquid of Lactobacillus rhamnosus into the above-mentioned filtrate medium, and culture it at 37°C.

[0033] Lentinus edodes mycelium is rich in amino acids, vitamins and minerals. The OD value of the culture medium in this example is 1....

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Abstract

The invention discloses a method for preparing an efficient lactic acid bacteria culture medium by liquid-state fermentation of shredded edible fungi. The method comprises the following steps: grinding a proper amount of edible mycelia subjected to liquid-state fermentation into slurry, adding water, and performing boiling, filtering, volume fixing and pH value regulating to obtain a filtrate culture medium, performing subpackaging, and performing sterilizing. The liquid lactic acid bacteria culture medium provided by the invention is low in cost and is simple to prepare, and rich protein andfree nucleotide in mycelia can effectively reduce the death number of lactic acid bacteria, delay the death time and avoid degradation and variation of thalli. Compared with a common MRS culture medium, the OD600 value is obviously superior to that of an MRS culture medium after the culture is performed for 18 hours, and the OD600 value of the finally optimized culture medium is 1.05 times or morethat of the common MRS culture medium.

Description

technical field [0001] The invention relates to a method for preparing a high-efficiency liquid culture medium for lactic acid bacteria from edible mycelia. Background technique [0002] Lactic acid bacteria are Gram-positive bacteria that can improve animal immunity, digestion and metabolism, and have strict nutritional requirements. When choosing a lactic acid bacteria medium, it should be conducive to the growth and rapid reproduction of lactic acid bacteria, and at the same time, it can properly improve the stress resistance of the bacterial cells. The culture medium used in industrial production must be low in cost, easy to obtain raw materials, and high in bacterial cell acquisition rate. Although there are many types of lactic acid bacteria culture medium, the basic culture medium can be roughly divided into four categories: whey protein medium, skimmed milk enzyme hydrolyzate medium, skim milk medium, and MRS milk enzyme hydrolyzate medium. MRS medium is suitable f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/25C12R1/225
CPCC12N1/20
Inventor 张永覃佐东何福林王宗成田思颖李志祥
Owner HUNAN UNIV OF SCI & ENG
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