Preparation of hydrophobic low-temperature co-melt and method for extracting Congo red, acidic golden yellow and red 2G from meat products and aquatic products

A low-temperature eutectic, hydrophobic technology, applied in the fields of food testing, aquatic product testing, and meat products, can solve the problems of cumbersome steps, respiratory tract damage, and large consumption of reagents, so as to simplify experimental steps, reduce testing costs, and reduce testing. cost effect

Pending Publication Date: 2020-09-18
甘肃省食品检验研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to: provide a kind of hydrophobic low-temperature eutectic preparation and the method for extracting Congo red, acid golden yellow and red 2G in meat product, aquatic product, to solve the above-mentioned traditional method existing loaded down with trivial details step, reagent consumption is big, easily Problems such as causing respiratory damage to personnel

Method used

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  • Preparation of hydrophobic low-temperature co-melt and method for extracting Congo red, acidic golden yellow and red 2G from meat products and aquatic products
  • Preparation of hydrophobic low-temperature co-melt and method for extracting Congo red, acidic golden yellow and red 2G from meat products and aquatic products
  • Preparation of hydrophobic low-temperature co-melt and method for extracting Congo red, acidic golden yellow and red 2G from meat products and aquatic products

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Embodiment 1

[0051] A method for preparing hydrophobic low-temperature eutectics. Weigh different hydrogen bond donors and hydrogen bond acceptors in 25mL round bottom flasks, and heat them in an oil bath at 55°C until the two mixtures become completely transparent and clear. The liquid is fine, let cool to room temperature and set aside. The pairing relationship between the hydrogen bond acceptor and the hydrogen bond donor synthesized by the hydrophobic low temperature fusion is shown in Table 2.

[0052] Table 2 Synthesis table of hydrophobic low temperature eutectic

[0053]

[0054]

Embodiment 2

[0056] On the basis of Example 1, a detection method for three illegally added industrial dyes in meat products and aquatic products comprises the following steps:

[0057] Step 1. Take a dry sample. The ratio of sample g to water mL is 1:5. Ultrasound for 10 minutes, centrifuge at 10,000 rpm for 5 minutes, and take the supernatant. The samples include meat products or aquatic products. For meat products, take the muscle part, and for aquatic products Take the skin and meat part, homogenize and dry to obtain a dry sample;

[0058] Step 2. Put 200 μL HDES and all supernatants into a 10 mL glass syringe, vortex for 3 minutes, and let it stand for 2 minutes to produce oily droplets. Pipette all the oily droplets into a 2mL capped centrifuge tube, dissolve with acetonitrile and make to volume to 1.0 mL, pass through a 0.45 μm microporous organic filter membrane to obtain the filtrate;

[0059] Step 3, the filtrate is determined by liquid mass spectrometry.

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Abstract

The invention discloses preparation of a hydrophobic low-temperature co-melt and a method for extracting Congo red, acid golden yellow and red 2G from meat products and aquatic products. The inventionbelongs to the technical field of food detection. In order to solve the problems of tedious steps, high reagent consumption, high possibility of causing respiratory tract injury to personnel and thelike in the traditional method, the method comprises the steps of mixing a hydrogen bond receptor and a hydrogen bond donor, heating until the mixture is completely changed into transparent clear liquid, and cooling to room temperature. The detection method comprises the steps of taking a dry sample, adding water for ultrasonic treatment, centrifuging and taking a supernatant; mixing, whirling andstanding the hydrophobic low-temperature co-melt and all the supernatant to generate oily liquid drops, sucking all the oily liquid drops, dissolving with acetonitrile, fixing the volume, and filtering with a microporous organic filter membrane to obtain a filtrate; and measuring the filtrate by using a liquid chromatograph-mass spectrometer. Under the condition that the detection rate is guaranteed, the detection efficiency is greatly improved, the detection cost is reduced, the test steps are greatly simplified, and the detection method is good in applicability and stability.

Description

technical field [0001] A method for preparing hydrophobic low-temperature eutectics and extracting Congo red, acid golden yellow and red 2G in meat products and aquatic products. The invention belongs to the technical field of food detection, and specifically relates to the technical field of detection of meat products and aquatic products. Background technique [0002] Because illegally added industrial dyes have the characteristics of low price, strong coloring, and strong stability, driven by economic interests, unscrupulous merchants add illegal industrial dyes in the production process of products to achieve the purpose of improving color and appearance, and then seek Benefit. According to the national food supervision sampling rules and risk monitoring tasks in recent years, the illegal addition of industrial dyes has attracted more and more attention, and Congo red in meat products has been listed as a testing item for monitoring. Azo dyes are formed by coupling diaz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88G01N2030/062
Inventor 周佳孙国威黄韡禹洁王丽君朱书强
Owner 甘肃省食品检验研究院
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