Differential multiplexing interference-enhanced double-optical-comb coherent Raman spectrum detection system and method

Abstract

The invention relates to a differential multiplexing interference-enhanced double-optical-comb coherent Raman spectrum detection system which comprises a double-optical-comb light source system, a local oscillation generation module, a coherent Raman microscopy module, a collection detection module and the like. The local oscillation generation module is used for filtering partial frequency of a double-optical-comb light source so that the double-optical-comb light source can be used as local oscillation to interfere with a subsequent anti-Stokes signal and a coherent Stokes signal respectively, the measured alternating current component intensity is enhanced, and the signal to noise ratio of a measured signal is improved; meanwhile, the difference between the anti-Stokes signal and the coherent Stokes signal of the opposite phase is calculated through a differential multiplexing measurement method, and a double-optical-comb coherent Raman oscillation signal with higher intensity can be obtained. According to the invention, the problems of low signal-to-noise ratio and low sensitivity in the conventional broadband coherent Raman spectrum detection technology are solved, the influence of a non-resonance background can be better removed while rapid measurement is realized, and the method has important significance in the application fields of high-sensitivity microscopic detection of biology, medicine and the like.

Description

Technical field [0001] The invention belongs to the technical field of spectrum detection, and particularly relates to a differential multiplexing interference-enhanced dual-comb coherent Raman spectrum detection system and method. Background technique [0002] Label-free, molecular-specific coherent Raman spectroscopy detection can effectively distinguish the chemical bonds of biological tissue molecules, and has sub-micron spatial resolution. It is used in cell metabolism research, cancer cell detection, myelin-related disease detection, There are a large number of applications in biomedicine such as in vivo drug tracking. Coherent anti-Stokes Raman spectroscopy (CARS) detection is an important direction in the development of current coherent Raman spectroscopy detection technology. It has strong signal strength and short signal integration time, and it detects anti-Stokes The frequency has no fluorescence interference. These advantages enable CARS technology to be fully studi...

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Problems solved by technology

[0004] Although the Fourier transform CARS measurement has the advantages of rapidity and high resolution, and this measurement method can easily remove the interference of non-resonant background signals, as mentioned above, in terms of the noise of the detection process and the degree of effective signal utilization, The signal-to-noise ratio of this method still has a lot of room for improvement.

The signal-to-noise ratio of coherent Raman spectroscopy directly affects the detection of low-concentration substances and weak Raman spectral lines. Although coherent Raman spectroscopy has played an important role in many fields, its application is still limited to the detection of high-intensity Raman Measurement of spectral lines (such as C-H bonds of lipids)

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