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Method for extracting isorhamnetin 3-0-rutinoside from suaeda salsa

A technology of Suaeda salsa and Rutinoside, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of low purity, high price, difficult extraction and purification, etc., and achieve a safe, simple, and broad extraction process. Market prospect and effect of extensive application value

Active Publication Date: 2020-09-29
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently isorhamnetin 3-O-rutinoside has problems such as low purity, small dose, high price, and difficulty in extraction and purification, which is not conducive to the clinical research of isorhamnetin 3-O-rutinoside

Method used

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  • Method for extracting isorhamnetin 3-0-rutinoside from suaeda salsa
  • Method for extracting isorhamnetin 3-0-rutinoside from suaeda salsa

Examples

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Embodiment 1

[0036] Such as figure 1 As shown, the method for extracting isorhamnetin 3-O-rutinoside from Suaeda salsa according to an embodiment of the present invention comprises the following steps:

[0037] S102, using 95% ethanol to reflux for 3 hours to extract Suaeda salsa seed extract;

[0038] S104, concentrating and filtering to obtain mother liquor a, concentrating and drying mother liquor a to obtain sample b;

[0039] S106, using A: water (0.1% TFA) B: acetonitrile as mobile phase, medium pressure preparative liquid phase separation to obtain sample c;

[0040] S108, using A: water (0.1% TFA) B: acetonitrile as mobile phase, preparative liquid phase separation to obtain sample d;

[0041] S110, using methanol as the mobile phase, gel (Sephadex LH-20) separation to obtain isorhamnetin 3-O-rutinoside. The structural formula of isorhamnetin 3-O-rutinoside is as follows figure 2 shown.

[0042] The collected Suaeda salsa seeds were soaked and pretreated before extraction.

Embodiment 2

[0044] S202, seed pretreatment: soak 550 g of Suaeda salsa seeds collected in ultrapure water for 24 hours, and dry at room temperature;

[0045] S204, extracting the seed extract: extracting Suaeda salsa seeds with 95% ethanol under reflux for 3 hours, concentrating to 1L-2L, filtering to obtain mother liquor a, concentrating and drying the obtained mother liquor a, and obtaining about 25 g of sample b;

[0046]S206, sample medium pressure preparation liquid phase separation: Dissolve sample b with a mixture of ethanol and tetrahydrofuran (1:1) and filter, use medium pressure preparation liquid phase separation, the liquid phase mode is Agilent Technologies971-FP FlashPurification System, chromatographic column Daiso ODS-C18 500×80mm, 40-60um, room temperature;

[0047] The mobile phase is ultrapure water (A) and acetonitrile solution (B) containing 0.1% (v / v) formic acid for gradient elution, the elution gradient is 0%-60% 60min, 60%-100% 25min, the flow rate is 70mL·min-1;...

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Abstract

The invention provides a method for extracting isorhamnetin 3-0-rutinoside from suaeda salsa. The method comprises the following steps of (1) performing soaking pretreatment on collected suaeda salsaseeds; (2) extracting suaeda salsa seed leach liquid through hot reflux with 95% ethanol, performing concentrating, and performing filtration to obtain mother liquid a; (3) concentrating and drying the mother liquid a, to obtain samples b; (4) dissolving the samples b with mixed liquid of ethanol and tetrahydrofuran, performing filtering, and separating prepared liquid phases with middle pressureto obtain fraction c; (5) dissolving the fraction c with mixed liquid of water and acetonitrile, performing filtering, and then separating the prepared liquid phases, to obtain fraction d; and (6) dissolving the fraction d with methanol, and performing gelatin separation by using the methanol as a mobile phase to obtain solid powder isorhamnetin 3-0-rutinoside. The technical scheme realizes the extraction of the isorhamnetin 3-0-rutinoside from the suaeda salsa, the extraction process is simple and convenient, and the purity can achieve 99.5%.

Description

technical field [0001] The invention relates to the technical field of natural medicine extraction, in particular to a method for extracting isorhamnetin 3-O-rutinoside from Suaeda salsa. Background technique [0002] Suaeda salsa (Suaeda salsa) belongs to Chenopodiaceae (Chenopodiaceae) annual herbaceous plants, aliases Salsa salsa, Suaeda winged, yellow mustard, widely distributed in North China, Northeast and Northwest China. As a traditional wild vegetable in the north, as early as the famine era, people used Suaeda salsa as food to satisfy their hunger. The stems and leaves of the plant are rich in nutrients, and the growth environment is far away from human habitation, and less polluted by chemical fertilizers and pesticides. It is a typical pollution-free green vegetable and is also a local specialty food. It is used for cold salad, stuffing, stir-fry Food etc. Due to its high nutritional value, the plant is used as a raw material for food and beverages in the food ...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07
Inventor 白军红王昕王伟张光亮王大伟
Owner BEIJING NORMAL UNIVERSITY
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