Application of compound in preparation of antiviral drug
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A compound and solvent compound technology, applied in the field of medicine, can solve problems such as no vaccine
Pending Publication Date: 2020-10-02
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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Problems solved by technology
However, there is still no effective preventive vaccine against HSV. Nucleoside analogues of lovir (such as acyclovir and ganciclovir) are generally used for treatment, and the replication of simple disease viruses is stopped in three ways: 1) Competitive inhibition of viral DNA polymerase; 2) entry and termination of elongated viral DNA strands; 3) inactivation of viral DNA polymerase
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Embodiment 1
[0047] Embodiment 1: the preparation of dihydromyricetin
[0048] (1) Get rattan tea (produced in Zhangping City, Fujian Province), pulverize, and cross a 50-mesh sieve to obtain rattan tea powder;
[0049] (2) Take vine tea powder, add 10 times the amount of 75% ethanol, ultrasonic for 30 minutes, filter, and filter to obtain dihydromyricetin extract;
[0050] (3) Place the above-mentioned dihydromyricetin extract in a rotary evaporator, concentrate under reduced pressure in a water bath at a temperature of 40°C, recover ethanol until it has no alcohol smell; add 5 times the amount of boiling water to dissolve, and place it in a low temperature environment of 4°C to cool Crystallize and filter to obtain the crude product of dihydromyricetin;
[0051] (4) Take the dihydromyricetin crude product and add 10 times the amount of boiling water to dissolve it, add activated carbon (0.5% of the crude product amount) to absorb for 20 minutes, suction filter while it is hot, let the f...
Embodiment 2
[0053] Example 2: Anti-herpes simplex virus HSV-1 activity experiment of dihydromyricetin
[0054] 1. Experimental materials
[0055] (1) Drug: Dihydromyricetin (purity is 99.5%; for example, can be prepared by the method of Example 1); Acyclovir (Aciclovir, ACV) (purchased by Selleck Chemical Company, batch number: S180701)
[0056] (2) Cells: Vero cells
[0057] (3) Virus: HSV-1 (provided by Wuhan Institute of Virology, Chinese Academy of Sciences)
[0059] (5) Main instruments: ultra-clean workbench (ThermoScientific), microplate reader (Bio-Tek Company), low-temperature high-speed centrifuge (Eppendorf Company), inverted microscope (Olympus Company), autoclave (Shanghai No. 5 Medical Device Factory) ), pure water meter (Milli...
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Abstract
The invention discloses an application of a compound in preparation of an antiviral drug. In one embodiment of the invention, the in-vitro anti-herpes simplex virus I activity of dihydromyricetin is verified through experiments, virus proliferation and related gene expression can be inhibited, the toxicity is relatively low, and a relatively high treatment index can be obtained. Meanwhile, it is proved that the anti-herpes simplex virus action mechanism of the dihydromyricetin is related to inhibition of TLR9-dependent inflammatory response pathways by the dihydromyricetin. In one embodiment of the invention, the dihydromyricetin is disclosed to have potential value of serving as the novel anti-herpes simplex virus drug, and can be applied to preparation of the anti-herpes simplex virus drug.
Description
technical field [0001] The invention belongs to the field of medicine, and specifically relates to the application of compounds in the preparation of antiviral drugs. Background technique [0002] Herpes simplex virus belongs to the subfamily Alphavirinae of the family Herpesviridae and is a DNA virus with a plasmid size of about 180 nanometers. The virus generally enters the body through the respiratory tract, genital mucosa and damaged skin, and resides in the normal mucosa, blood, saliva and sensory ganglion cells of the human body. More than 90% of the population has been infected with herpes simplex virus. When the body's resistance declines, the latent herpes simplex virus in the body is activated to cause the disease. According to the difference in antigenicity, the virus is currently divided into type I and type II, and humans are its only host. Type I herpes simplex virus (HSV-1) mainly infects the skin and mucous membranes of the face, causing herpes labialis, he...
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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/353A61P31/22A61P31/16A61P31/14
CPCA61K31/353A61P31/22A61P31/16A61P31/14
Inventor 方建国周海云施春阳王文清
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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