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Method for improving resistance of plant to fusarium graminearum by utilizing gene AtALA1

A technology of Fusarium graminearum and transgenic plants, applied in the field of plant genetic engineering

Active Publication Date: 2020-10-02
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, most researchers mainly use the former method to achieve the purpose of improving plant resistance, and there are no reports on the successful use of foreign gene expression products to detoxify the toxins produced by pathogenic bacteria to improve resistance to pathogenic bacteria.

Method used

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  • Method for improving resistance of plant to fusarium graminearum by utilizing gene AtALA1
  • Method for improving resistance of plant to fusarium graminearum by utilizing gene AtALA1
  • Method for improving resistance of plant to fusarium graminearum by utilizing gene AtALA1

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Embodiment Construction

[0070] The present invention will be described in further detail below in conjunction with the accompanying drawings, but the following description does not limit the present invention.

[0071] The pharmaceutical reagents in the implementation examples of the present invention are domestic conventional chemical reagents that are not specified, and all materials and methods that are not specified are referred to "Molecular Cloning Experiment Guide" (Sambrook and Russell, 2001).

[0072] 1. Proposal of the core strategy of the invention technology

[0073] 1.1 Obtaining homozygous mutants of Arabidopsis thaliana with deletion of AtALA1 gene

[0074] 1.1.1 Arabidopsis mutant acquisition and planting

[0075] Arabidopsis mutants were purchased from Arabidopsis Biological Resource Center (Arabidopsis Biological ResourceCentre, ABRC) T-DNA insertion mutants, numbers are: ala1 (SALK_056947), ala3 (SALK_082157), ala6 (SALK_150173), ala7 (SALK_125598 ), ala10 (SALK_024877).

[0076...

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Abstract

The invention relates to a method for improving the resistance of a plant to fusarium graminearum. The method comprises the following steps of integrating a gene AtALA1 into the target plant, and expressing the gene AtALA1 in the target plant to reduce the toxin content of the fusarium graminearum so as to improve the resistance of the plant to the fusarium graminearum, wherein a nucleotide sequence of the gene AtALA1 is as shown in SEQ ID NO.14. According to the method, the toxin content in the plant body is obviously reduced and the resistance to the fusarium graminearum is improved by utilizing a transport degradation mechanism of the plant to toxins of pathogenic bacteria. Therefore, the method can be widely used for improving the resistance of different pathogenic bacteria and toxinsthereof, and has wide applicability.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering. Specifically, it relates to a method for improving plant resistance to Fusarium graminearum by using genetic engineering technology to reduce the content of Fusarium graminearum toxin. Background technique [0002] Rice and corn are the world's three major food crops and the main sources of food and feed in the world. The safety of their products is directly related to the survival of human beings. However, these cereal crops are often infected by a variety of pathogenic bacteria during their growth and development, which not only seriously reduces their yield and quality, but the grains contaminated by pathogenic bacteria often contain a variety of mycotoxins, which seriously endanger human and animal health (Zhen, 2010). According to the United Nations Food and Agriculture Organization (FAO), as many as 25% of the world's food crops are contaminated with mycotoxins, of which deoxynival...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/29A01H5/00A01H6/20A01H6/46
CPCC12N15/8282C07K14/415
Inventor 裴炎王凡龙侯磊韩菁卓静欣苏梅李玉杰任慧宋水清
Owner SOUTHWEST UNIVERSITY
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