Detection method of active ingredients in Wuling Capsules
A detection method and technology for active ingredients are applied in the field of detection of active ingredients in Wuling Capsules, which can solve the problems of inaccurate detection methods of Wuling Capsules, achieve strict and standardized production process, good repeatability, and ensure stability and safety. Effect
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[0028]The preparation process of the Bupleurum radix sample solution to be tested can adopt the methods commonly used in the art. In a preferred embodiment, the preparation process of Bupleurum bupleurum test sample solution includes: mixing Wuling Capsules with water, and filtering to obtain the first filtrate; using the first extractant and the second extractant in sequence for the first filtrate Extraction is carried out to obtain an extract; the extract is washed, and then evaporated to dryness to obtain the first solid residue; the first solid residue is dissolved by the first organic solvent to obtain a sample solution of Bupleurum bupleuri; the first extractant includes but is not limited to n-butyl Saturated aqueous solution of alcohol; the second extractant includes but not limited to ammoniation test solution; the first organic solvent includes but not limited to methanol. Since each component in Wuling Capsules has good water solubility, it is now transferred to an ...
Embodiment 1
[0044] Preparation of the sample solution to be tested:
[0045] Take 2g of the contents of Wuling Capsules, add 30mL of water to dissolve, shake for 30min, filter, extract the filtrate twice with water-saturated n-butanol, 20mL each time, combine n-butanol, and extract twice with ammonia test solution, 20mL each time , Discard the ammonia test solution, then wash twice with n-butanol-saturated water, 20 mL each time, discard the washing solution, evaporate the n-butanol solution to dryness, add 1 mL of methanol to the residue to dissolve, and use it as the test solution.
[0046] Preparation of the reference medicinal material solution: take 1 g of the reference medicinal material of Bupleurum chinensis, and prepare the reference medicinal material solution by the same preparation method as the sample solution to be tested.
[0047] Preparation of Negative Control Solution:
[0048] Take 2 g of the negative control substance, and make a negative control solution with the pre...
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