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A characteristic type III collagen peptide derived from ducks and its application in the detection of collagen hydrolyzate and its products

A collagen hydrolyzate, collagen peptide technology, applied in specific peptides, biological testing, peptides, etc., can solve problems such as insurmountable difficulties, and achieve a highly conservative effect

Active Publication Date: 2022-06-21
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned difficulties cannot be overcome by directly using liquid mass spectrometry to detect and analyze samples.

Method used

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  • A characteristic type III collagen peptide derived from ducks and its application in the detection of collagen hydrolyzate and its products
  • A characteristic type III collagen peptide derived from ducks and its application in the detection of collagen hydrolyzate and its products
  • A characteristic type III collagen peptide derived from ducks and its application in the detection of collagen hydrolyzate and its products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1 Materials and Reagents

[0020] Materials: Gelatin from different animal sources is derived from chicken, duck, cow, pig, and sheep, and is extracted from chicken skin, duck skin, cowhide, pigskin, and sheepskin.

[0021] Reagents: ammonium bicarbonate (analytical grade), trypsin (sequence pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products).

[0022] 2 Screening and determination of characteristic collagen peptides

[0023] The detection of duck skin shows that duck skin contains a large amount of COL3A1 protein, and this protein has a certain degree of conservation, so a peptide can be screened from COL3A1 protein as a characteristic type III collagen peptide of duck origin. In order to screen out stable characteristic collagen peptides for detection from genes, the following conditions should be met: (a) The three bases adjacent to the mRNA sequence corresponding to the peptide should be arginine or lysine after translati...

Embodiment 2

[0032] 1 Materials and Reagents

[0033] Materials: Mixed gelatin samples (including: porcine gelatin with 5% duck gelatin, porcine gelatin with 10% duck gelatin, porcine gelatin with 20% duck gelatin, porcine gelatin with 40% duck gelatin, porcine gelatin with 60% duck gelatin, pure Duck Gelatin) was prepared from the exact mixing of the gelatin from Example 1.

[0034] Reagents: ammonium bicarbonate (analytical grade), trypsin (sequence pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products).

[0035] 2 Detection methods

[0036] (1) Take 0.05g gelatin sample into a 25ml measuring bottle, add a small amount of 1% NH 4 HCO 3 The solution was soaked for 10min, heated at 60°C to dissolve it, and dissolved with 1% NH 4 HCO 3 The solution was diluted to the mark, shaken well, filtered through a microporous membrane, 0.2 ml of the subsequent filtrate was precisely measured, 20 μl of 2 mg / ml trypsin solution was added, and 37 °C constant...

Embodiment 3

[0040] 1 Materials and Reagents

[0041] Material: duck gelatin, QQ sugar added with bovine gelatin (homemade), QQ sugar added with duck gelatin (homemade);

[0042] Reagents: ammonium bicarbonate (analytical grade), trypsin (sequence pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products).

[0043] 2 Detection methods

[0044] (1) Take 0.05-0.25g of duck gelatin, QQ sugar added with bovine gelatin, and QQ sugar added with duck gelatin, put them in a 25ml measuring bottle respectively, add a small amount of 1% NH 4 HCO 3 The solution was soaked for 10min, heated at 60°C to dissolve it, and dissolved with 1% NH 4 HCO 3 The solution was diluted to the mark, shaken well, filtered through a microporous membrane, 0.2 ml of the subsequent filtrate was precisely measured, 20 μl of 2 mg / ml trypsin solution was added, and 37 °C constant temperature enzymatic hydrolysis was carried out for 12 h.

[0045] (2) Take 5 μl of enzymolysis solution ...

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Abstract

A characteristic type III collagen peptide derived from ducks and its application in the detection of collagen hydrolyzate and its products belong to the technical field of detection. The present invention relates to the characteristic type III collagen peptide Gly-Ser-Hyp-Gly-Pro-Hyp-Gly-Pro-Ser-Gly-Pro-Pro-Ala-Gly-Glu-Arg of duck origin, which can be detected by detecting the characteristic type III Collagen peptide, to determine whether it contains duck-derived ingredients. Specifically, trypsinize the protein components in the sample to free the characteristic type III collagen peptide, and then detect whether it contains the characteristic type III collagen peptide by liquid chromatography-mass spectrometry to determine whether it contains duck-derived sexual component. The method has strong characteristics, high sensitivity and simple operation, and can determine duck-derived components in collagen hydrolyzate and its products.

Description

technical field [0001] The invention relates to a characteristic type III collagen peptide of duck origin and its application in the detection of collagen hydrolyzate and its products, belonging to the field of medicine and food detection. Background technique [0002] Collagen is very abundant in animals and mainly exists in connective tissues such as bones, skins, tendons and tendons of animals. Gelatin is a degradation product of collagen. It is obtained from animal bones, skins, tendons and tendons through moderate hydrolysis. It has excellent physical and chemical properties and is widely used in many fields such as food and medicine. Most of the gelatin is made from the skins or bones of pigs, cattle and sheep. However, due to the safety risks, halal and religious beliefs that may be brought about by diseases of poultry and livestock, it is of great significance to trace the animal-derived components of gelatin. [0003] The main component of gelatin is collagen polyp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08
CPCG01N30/06G01N30/72G01N30/8634G01N30/88G01N30/89G01N30/14C07K14/78C12P21/06G01N33/68G01N2030/067G01N2030/8831G01N2030/8818G01N2030/146G01N2333/78
Inventor 郭尚伟黄雅钦
Owner BEIJING UNIV OF CHEM TECH
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