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Preparation method and application of calprotectin biosensor based on graphene

A technology of biosensor and calprotectin, which is applied in biological testing, material inspection products, material electrochemical variables, etc., can solve the problems of long time, low sensitivity, and many detection steps, and achieve the effect of sensitive detection

Pending Publication Date: 2020-10-09
INST OF METAL RESEARCH - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

ELISA method is widely used in hospital inspection due to its quantitative accuracy, but this method has the disadvantages of many detection steps, long time and inconvenient; although colloidal gold method is convenient and fast, it is only a qualitative method with low sensitivity

Method used

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  • Preparation method and application of calprotectin biosensor based on graphene
  • Preparation method and application of calprotectin biosensor based on graphene

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Embodiment 1

[0045] In the present embodiment, the preparation method and application thereof of the graphene-based calprotectin biosensor are as follows:

[0046] 1. Modification of Calprotectin Immunosensor Electrodes

[0047] (1) prepare the phosphate (PBS) dispersion liquid that concentration is 1mg / ml graphene oxide and the chitosan acetic acid solution that concentration is 0.2wt% respectively.

[0048] (2) Modification of the polished electrode surface

[0049] First, 1 μL of the above-mentioned graphene oxide dispersion was added dropwise on the electrode, and after the electrode was dried at room temperature, 5 μL of chitosan acetic acid solution with a concentration of 0.2 wt% was added dropwise. After the electrode is dry, put the above-mentioned modified electrode into PBS with a concentration of 0.1mol / L, and perform cyclic voltammetry scanning in the voltage range of 0-1.5V for 10 weeks to reduce the graphene oxide on the electrode to graphene . Prepare a PBS solution cont...

Embodiment 2

[0055] The modification method of the calprotectin immunosensor electrode is the same as in Example 1, except that the modified amount of calprotectin on the electrode is 40 ng / mL, and the test method is also the same as in Example 1, and the corresponding peak current on the DPV curve is 38.3 μA.

[0056] Such as figure 1 As shown, it can be seen from the DPV curves of electrodes modified with different concentrations of calprotectin that as the concentration of modified calprotectin increases, the peak current on the corresponding DPV curve decreases significantly. This is because calprotectin, as a biologically active substance, has no electrical activity, and the higher the concentration of modified calprotectin on the electrode, the greater the impedance of the corresponding electrode, making the peak current on the DPV curve lower. The peak current on the DPV curve changes regularly with the concentration of calprotectin, which is a prerequisite for the electrochemical d...

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Abstract

The invention belongs to the field of immunosensors, and particularly relates to a preparation method and application of a calprotectin biosensor based on graphene. Firstly, a cleaned sensor electrodeis coated with graphene oxide dispersion liquid in a dropwise mode, then the surface of the electrode is coated with a chitosan acetic acid solution in a dropwise mode, graphene oxide is converted into graphene through electrochemical reduction, and calprotectin antibodies are easily enriched and fixed to the surface of the electrode through biocompatibility of the graphene and carboxyl on the surface of the graphene. The electrode is activated, a calprotectin antibody is fixed, an active site is closed, and finally calprotectin is fixed on the electrode through immunoreaction of an antigen and an antibody. As the peak current of the electrode with excellent conductivity of graphene in the electrochemical probe potassium ferricyanide solution is obviously increased, the sensitivity of theimmunosensor can be remarkably improved. The excellent biocompatibility of the graphene can effectively improve the modification amount of the calprotectin antibody on the surface of the electrode, so that the detection range of the electrode on the calprotectin concentration is enlarged.

Description

technical field [0001] The invention belongs to the field of immune sensors, in particular to a preparation method and application of a graphene-based calprotectin biosensor. Background technique [0002] Colorectal cancer is one of the common clinical malignant tumors, and has a high incidence rate in digestive system tumor diseases. With the increasingly serious environmental pollution and food safety problems and the acceleration of people's life rhythm, the dietary structure has changed to varying degrees, coupled with the development of population aging and the weakening of physical functions, making the elderly a high-incidence group of colorectal cancer. In recent years, the incidence of this disease has shown a trend of increasing significantly, seriously affecting the health of the public. Early detection, early diagnosis, and early treatment are of great significance for prolonging the survival time of patients, improving patient prognosis and reducing mortality. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N27/48
CPCG01N33/68G01N27/48
Inventor 英哲张睿杨卫东郝亚斌孙宝明刘放王永鹏曾尤成会明
Owner INST OF METAL RESEARCH - CHINESE ACAD OF SCI
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