Sodium lauroyl sarcosine-glycylglycine compound and composite oil displacement agent
A technology of double glycoside peptide compound oil displacement agent, sodium lauroyl sarcosinate, applied in drilling composition, peptide, organic chemistry and other directions, can solve the problem of reducing the effect of microbial oil displacement, inability to achieve microbial oil displacement, and microbial movement. It can improve the efficiency of synergistic reaction, the operability and applicability are strong, and the method is simple.
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Embodiment 1
[0069] This embodiment provides a kind of microbial-chemical composite oil displacement agent, and it is prepared through the following steps:
[0070] (1) Add 200mL of the culture medium of the inoculated strain to a 500mL Erlenmeyer flask, place it in an air-bath shaker at 65°C and 170r / min for cultivation, and when the bacteria grow to the late logarithmic period, centrifuge at 8000r / min for 15min to collect the cells , washed with dilute sulfuric acid (pH value = 2) to remove impurities, and suspend the bacteria to make a suspension (1cm cuvette) with an absorbance value of 1 at a wavelength of 600nm. Centrifuge for 10 minutes to collect the lower layer of bacteria;
[0071] (2) Bacteria were eluted with water at 40°C for 12h, centrifuged, and the upper capsule solution was taken for chromatographic separation and purification to obtain the diglycopeptide (during the chromatographic separation, the retention time of the upper capsule solution was determined according to th...
Embodiment 2
[0078] This embodiment provides a kind of microbial-chemical composite oil displacement agent, and it is prepared through the following steps:
[0079] (1) Add 200mL of the culture medium of the inoculated strain to a 500mL Erlenmeyer flask, place it in an air-bath shaker at 65°C and 170r / min for cultivation, and when the bacteria grow to the late logarithmic period, centrifuge at 8000r / min for 15min to collect the cells , washed with dilute sulfuric acid (pH value = 2) to remove impurities, and suspend the bacteria to make a suspension (1cm cuvette) with an absorbance value of 1 at a wavelength of 600nm. Centrifuge for 10 minutes to collect the lower layer of bacteria;
[0080] (2) Bacteria were eluted with water at 40°C for 12h, centrifuged, and the upper capsule solution was taken for chromatographic separation and purification to obtain the diglycopeptide (during the chromatographic separation, the retention time of the upper capsule solution was determined according to th...
Embodiment 3
[0084] This embodiment provides a kind of microbial-chemical composite oil displacement agent, and it is prepared through the following steps:
[0085] (1) Add 200mL of the culture medium of the inoculated strain to a 500mL Erlenmeyer flask, place it in an air-bath shaker at 65°C and 170r / min for cultivation, and when the bacteria grow to the late logarithmic period, centrifuge at 8000r / min for 15min to collect the cells , washed with dilute sulfuric acid (pH value = 2) to remove impurities, and suspend the bacteria to make a suspension (1cm cuvette) with an absorbance value of 1 at a wavelength of 600nm. Centrifuge for 10 minutes to collect the lower layer of bacteria;
[0086] (2) Bacteria were eluted with water at 40°C for 12h, centrifuged, and the upper capsule solution was taken for chromatographic separation and purification to obtain the diglycopeptide (during the chromatographic separation, the retention time of the upper capsule solution was determined according to th...
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