Poplar Melampsora laricipopulina single spore propagation method

Abstract

The invention discloses a poplar Melampsora laricipopulina single spore propagation method, and belongs to the technical field of microbial cultivation. The method provided by the invention comprisessteps of seedling obtaining, infected leaf collection and strain primary propagation, single pore system transfer, expanding propagation and the like. The method comprises the steps of using a 90 mm disposable culture vessel; padding two pieces of filter paper at the bottom of the culture vessel; adding 3 mL of sterile water for moisture preservation; dispersing single sori on infected leaves into4 [mu]L of an agar water solution; spraying a solution mixed uredinium onto tender leaves of NL895 with the disease infection capability; after inoculation is completed, putting the materials into agreenhouse with the temperature of 19 DEG C and the light period of 16 h / 24 h to be cultured; after the morbidity, performing further propagation to the sufficient quantity; and performing storage ina refrigerator of 4 DEG C for use. An indoor inoculation method is used; the Melampsora laricipopulina is cultured by a leaf disc method; the separated sori can be obtained; the method is simple and efficient; and purified and single stains are provided for better studying the poplar rust resistance.

Description

technical field [0001] The invention belongs to the technical field of microorganism culture, in particular to a single-spore propagation method for poplar and larch poplar rust fungus (Melampsora laricipopulina). Background technique [0002] Poplar is widely cultivated all over the world. Because of its fast growth, strong adaptability, many varieties and short breeding time, poplar has become the main tree species in windbreak and sand fixation forests, farmland shelter forests, soil and water conservation forests and timber forests. However, with the development of large-scale poplar plantations, many diseases are prevalent thereupon, seriously hindering the growth of poplar. In particular, the occurrence of poplar leaf rust caused the disease to spread rapidly in poplar forests and brought huge losses to poplar plantations. A species of P. larix that causes poplar leaf rust is a serious pathogenic fungus on hybrid poplars in the world. Compared with mature trees, seedl...

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Problems solved by technology

And it can only survive and reproduce on living poplar leaves, and it is difficult to propagate monospores, and there is no report on the propagation of monospores of Larix poplarus at home and abroad.

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