Application of pseudomonas putida in prevention and treatment of crop weeds
A technology of Pseudomonas putida and crops, applied in the agricultural field, can solve problems such as blanks, and achieve the effect of simple application and environmental friendliness
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Embodiment 1
[0023] Effect of Pseudomonas putida on germination rate of weed seeds
[0024] Take 1500 mL OD 600 Pseudomonas putida cells with an absorbance value of about 1.5 were centrifuged at 4°C and 8000 rpm for 20 min to collect Pseudomonas putida cells. Add 10 mL of pure water to the cell pellet, gently suspend and wash the cells, repeat once, and remove the residual LB liquid medium. Add 10 mL of pure water to the cell pellet, and gently resuspend the cell. Take 1.3 mL, 1 mL, 0.6 mL, 0.4 mL, 0.3 mL, 0.2 mL, 0.05 mL, 0.02 mL, and 0.01 mL of resuspended bacteria, respectively, and transfer them to 100 mL of pure water, mix well, and set Water was used as a negative control. Put 50 seeds of crabgrass seeds, Henyagrass seeds, Striga seeds, indica rice seeds, and featherweed seeds on floats containing gauze, put the floats into bacterial solutions with different concentrations, and cultivate them in a biochemical incubator at 37°C , the sunshine time is 12 h, on the second or third d...
Embodiment 2
[0027] Effects of Pseudomonas putida on germination rate of crabgrass seeds in rice seedling soil
[0028] Take 60 g of soil that has been sterilized by moist heat at 121°C, add 60 mL of pure water containing 75 million Pseudomonas putida cells to it, set the pure water without bacterial cells as a negative control, stir evenly, and put the soil Transfer to a straight cylindrical container with a diameter of 15 cm, and insert 15 rice seedlings at the second-leaf stage therein. Then evenly sprinkle 120 crabgrass seeds in the rice seedlings, place them outdoors for 3 days, pick out the crabgrass seeds, and calculate the germination rate.
[0029] The result is as figure 2 As shown in A, the germination rate of crabgrass seeds in the negative control (Control) was 63.9%, which contained Pseudomonas putida ( P. putida treatment) soil, the germination rate of crabgrass seeds was 10.8%, and the germination rate was significantly lower than that of the negative control. If the ge...
Embodiment 3
[0031] Effects of Pseudomonas putida on germination rate of crabgrass seeds in soybean soil
[0032] Take 60 g of soil sterilized by moist heat at 121 °C, transfer the soil to a straight container with a diameter of 15 cm, bury 3 soybean seeds at a depth of about 3 cm from the soil surface, and add an appropriate amount of sterilized water to the sowing place, Keep the soil moist. Cultivate in a biochemical incubator at 20°C until soybeans germinate. Sprinkle 20 mL of pure water containing 75 million Pseudomonas putida cells evenly into the soil, and set the pure water without bacteria as a negative control. Then 120 crabgrass seeds were evenly sprinkled into the soil, and after being placed outdoors for 3 days, the crabgrass seeds were picked out to calculate the germination rate.
[0033] The result is as image 3 As shown, the germination rate of crabgrass seeds in the negative control (Control) was 60.7%, which contained Pseudomonas putida ( P. putida treatment) soil,...
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