Biological manufacturing method of bionic vascular smooth muscle layer

Abstract

The invention provides a biological manufacturing method of a bionic vascular smooth muscle layer. A special-shaped needle head with parallel grooves in the lower portion is adopted. The outer wall ofmicrofilament blood vessels is printed through a 3D DIW ink direct writing technology. Through cell adhesion and further culture, a highly bionic smooth muscle layer is obtained in vitro. Most smoothmuscle cells are arranged in a circumferential shape, and the arrangement orientation and the cell phenotype of the smooth muscle cells are highly consistent with that of in-vivo vascular smooth muscle cells. The in-vitro model of the artificial blood vessel smooth muscle layer has the advantages of high bionics, customized production, adjustable thickness and the like, so that researchers can conveniently research the functions of the vascular smooth muscle layer in the human body in the future, and important pathogenesis of atherosclerosis, hypertension, heart failure, cerebral infarction,vascular aneurysm and other diseases caused by functional failure of the artificial blood vessel function failure. Meanwhile, a technical basis can be provided for producing the artificial blood vessel substitute capable of simulating the functional characteristics of natural blood vessels in the future.

Description

technical field [0001] The invention belongs to the field of 3D printing and biotechnology, and in particular relates to a bionic biomanufacturing method of vascular smooth muscle layer. Background technique [0002] Vascular smooth muscle cells (VSMCs) are multifunctional mesenchymal cells derived from the mesoderm during embryonic development surrounded by an extracellular matrix. VSMCs are the main components of the media of large and medium arteries, and are an important factor in maintaining the integrity of the vessel wall and maintaining the tension of the vessels, and can regulate blood pressure and blood flow. The phenotypic transformation caused by abnormal proliferation and migration of VSMCs plays a key role in cardiovascular and cerebrovascular diseases, and is one of the important pathological processes of diseases such as atherosclerosis, hypertension, heart failure, cerebral infarction and vascular aneurysm. At present, due to the lack of methods to restore ...

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Problems solved by technology

However, the stochastic nature of fiber deposition during electrospinning results in an isotropic surface that lacks the necessary spatial cues to guide cell elongation, resulting in inability to obtain a contractile phenotype similar to that seen in vivo in smooth muscle cells.

[0007] The vascular smooth muscle layer manufactured by the existing technology: the manufacturing process is complicated, and it is difficult to carry out large-scale production; most of the fiber materials that can be used are synthetic materials that are difficult to prepare (such as synthetic fibrin); the thickness of blood vessels is not adjustable; the deposited fibers Poor parallel orientation and width uniformity; difficult to keep all vascular structures consistent topographically when required to fabricate vascular structures for research multiple times

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