Preparation method and application of dandelion leaf water-soluble crude extract

A dandelion leaf and water-soluble technology, which is applied in the fields of application, medical preparations containing active ingredients, animal feed, etc., can solve the problems of complex operation, low extraction rate of water extraction technology, and lack of application technology, and achieve high content of active ingredients , excellent anti-inflammatory activity, and simple steps

Pending Publication Date: 2020-10-23
COASTAL AGRI RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above-mentioned known technologies mainly include dandelion organic solvent extraction and water extraction technology. Organic solvent extraction can increase the extraction rate to a certain extent, but it requires processes such as macroporous resin adsorption and elution, microporous membrane filtration and impurity removal, not only The operation is complicated, the preparation cost is high, and there is a risk of organic solvent residue, which does not really solve the safety problem required for production; the extraction rate of water extraction technology is low, the main active ingredient content of the obtained extract is low, and caffeic acid is only 0.8% ( CN105287682A), the phenolic acid active substance is only 1.59% (CN103445173A)
Moreover, the patented technology of dandelion extract is mainly used in the field of traditional Chinese medicine production, and the application technology in the large-scale production of alternative antibiotic feed is lacking

Method used

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  • Preparation method and application of dandelion leaf water-soluble crude extract
  • Preparation method and application of dandelion leaf water-soluble crude extract
  • Preparation method and application of dandelion leaf water-soluble crude extract

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preparation example Construction

[0035] The embodiment of the present invention provides a method for preparing a water-soluble crude extract of dandelion leaves, comprising the following steps:

[0036] (1) Water extraction pretreatment: add dandelion in cellulase solution, soak, obtain the dandelion of pretreatment; Described dandelion leaf is to wash the leaves of freshly picked dandelion dandelion, cut into sections 3 ~ 5cm, first in Lose 20% to 40% of water in a cool place, then dry in the sun or bake in an oven at 60-75°C for 4-6 hours; the cellulase is 0.1% cellulase, and the enzyme activity of the cellulase is 5000 ~10000U / g; the mass volume ratio of the dandelion leaf and the cellulase solution is 1g:(5mL~10mL), the pH value is adjusted to 4, the soaking temperature is 50~80°C, and the time is 6~12h;

[0037] (2) Water leaching: the pretreated dandelion is subjected to multiple water leaching, the leaching solution is combined, and the extracting solution is obtained after filtering; the pressure of ...

Embodiment 1

[0043] Embodiment 1 of the present invention discloses a kind of preparation method of dandelion water extract, specifically comprises the following steps:

[0044] (1) Cut the washed leaves of "Binpu No. 1" into 4cm sections, lose 25% of the water in a cool place, and then dry them in the sun or bake them in an oven at 65°C for 6 hours to obtain dandelion leaves; then add dandelion leaves according to 1g of dandelion leaves Add 6mL of 0.1% cellulase solution to the cellulase solution for soaking at 60°C for 8 hours;

[0045] (2) The negative pressure conditions in the water extraction process are: -0.05MPa, the temperature is 80°C, add 10 times the amount of water of the pretreated dandelion for the first extraction, and the time is 4 hours, and add the first extraction for the second extraction. Add 6 times the amount of water to the extract for 3 hours, add 2 times the amount of water to the second extract for 2 hours, combine the first extract, the second extract and the t...

Embodiment 2

[0048] Embodiment 2 of the present invention discloses a kind of preparation method of dandelion water extract, specifically comprises the following steps:

[0049] (1) Cut the washed leaves of "Binpu No. 1" into 4cm sections, lose 25% of the water in a cool place, and then dry them in the sun or bake them in an oven at 65°C for 6 hours to obtain dandelion leaves; add 6mL of 0.1% fiber to 1g of dandelion leaves The proportion of the enzyme solution is added to the cellulase solution for soaking, the soaking temperature is 60°C, and the soaking time is 8h;

[0050] (2) The negative pressure conditions for extraction are: -0.05MPa, temperature is 85°C, add 10 times the amount of water of pretreated dandelion for the first extraction, and the time is 4h, and add the first extraction for the second extraction 6 times the amount of water for 3 hours, add 2 times the amount of water for the second extraction for 2 hours, and combine the first extract, the second extract and the thir...

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Abstract

The invention discloses a preparation method of a dandelion leaf water-soluble crude extract. The preparation method comprises the steps of water extraction pretreatment, water extraction, concentration, spray drying and the like. The whole process is simple, the extraction rate is high, and the extraction cost is low; the prepared dandelion water-soluble crude extract is high in content of effective components, and can be used for preparing a feed additive capable of improving immunity and antisepsis and anti-inflammation activity. Wherein the extraction rate of the water-soluble crude extract can reach 27.4%, and is increased by 2 times compared with the extraction rate of 13.7% of the crude extract in the current market; and the content of caffeic acid is increased from 0.02% to 1.1% orabove.

Description

technical field [0001] The invention relates to the technical field of extraction of active ingredients in plants, and more specifically relates to a preparation method of dandelion water extract. Background technique [0002] Dandelion (Taraxacum mongolicum Hand.-Mazz.), also known as mother-in-law ding, huanghuamiao, huanghuadiding, huanghualang, wood yam, thin paste, patch, milk or bitter clover, etc. It is widely distributed in many places in North China, Central China, East China, and Northeast China. Dandelion has long been recorded in ancient books on the treatment of diseases. Among them, Li Shizhen's "Compendium of Materia Medica" clearly records: women's mastitis is swollen, boiled sweat, drink it and seal it, and it will disappear immediately. ; Detoxification, dissipating stagnant qi, detoxifying heat, reducing malignant swelling, tuberculosis, curing swelling, strengthening muscles and bones; applying white juice to malignant thorns and fox urine prickly sores w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/288A61P29/00A61P31/04A61P37/04A61P31/10A23K10/30A23K50/75A61K127/00
CPCA61K36/288A61K2236/10A61K2236/19A61K2236/331A61K2236/51A23K10/30A23K50/75A61P29/00A61P31/04A61P31/10A61P37/04
Inventor 李赵嘉吴哲王秀萍薛志忠张晓东杨雅华鲁雪林
Owner COASTAL AGRI RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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