Method for detecting concentration of antidepressant drugs in serum by using ultra-high performance liquid chromatography-tandem mass spectrometry technology

Abstract

The invention discloses a method for detecting the concentration of antidepressant drugs in serum by an ultra-high performance liquid chromatography-tandem mass spectrometry technology. The antidepressant drugs comprise bupropion, agomelatine, hydroxybupropion, nortriptyline, o-desmethylvenlafaxine, mianserin, mirtazapine, venlafaxine, amitriptyline, doxepin, norfluoxetine hydrochloride, duloxetine, fluoxetine, fluvoxamine, citalopram, paroxetine, trazodone and vortioxetine. After a serum sample is pretreated, a to-be-detected substance is separated from a serum matrix by utilizing ultra-highperformance liquid chromatography, a calibration curve is established by utilizing a mass spectrum isotope internal standard quantitative method and taking a concentration ratio of the standard substance to an internal standard substance as an X axis and a peak area ratio of the standard substance to the internal standard substance as a Y axis, and the content of the drugs in serum is calculated.The method is high in sensitivity, high in specificity, accurate and simple in pretreatment process, separation and detection can be completed within 4.5 min, and the accuracy degree and precision basically meet the requirements.

Description

technical field [0001] The invention belongs to the technical field of blood detection, and in particular relates to a method for detecting the concentration of antidepressant drugs in serum by ultra-high performance liquid chromatography tandem mass spectrometry. Background technique [0002] At present, the commonly used drugs for the treatment of depression are divided according to their mechanism of action: norepinephrine reuptake inhibitors, serotonin (5-HT) reuptake inhibitors (SSRIs), 5-HT and norepinephrine reuptake inhibitors SNRIs, norepinephrine and specific serotonergic antidepressants (NaSSAs), 5-HT2 receptor antagonists and 5-HT reuptake inhibitors, norepinephrine and dopamine reuptake inhibitors and melatonin receptor agonists. [0003] The methods for determining antidepressants in human serum or plasma mainly include HPLC-UV method, GC-MS method and LC-MS / MS method in domestic and foreign literature reports, such as the literature "Quantification of 33antid...

AI Technical Summary

Problems solved by technology

[0003] The methods for determining antidepressants in human serum or plasma mainly include HPLC-UV method, GC-MS method and LC-MS / MS method in domestic and foreign literature reports, such as the literature "Quantification of 33antidepressants by LC-MS / MS—comparative validation in whole blood, plasma and serum" reported a liquid-liquid extraction method for the detection of 33 antidepressants, but the pretreatment requires complex procedures such as liquid-liquid extraction, nitrogen blowing and concentration, and each sample collection takes 30 minutes

For example, Chinese patent (CN108982714A) discloses "a simple and efficient method for detecting the concentration of venlafaxine and its active metabolite o-desvenlafaxine in human plasma". Liquid extraction, then nitrogen blowing, reconstitution and sample injection, and then detect the concentration of the analyte by high performance liquid phase fluorescence method; the pretreatment of this invention is time-consuming and laborious, the amount of sample is large, and the analysis time of a single sample is longer

In addition, Chinese patent (CN 109655568A) discloses a "method and kit for simultaneous determination of 35 psychotropic drugs by HPLC-MS". Although the pre-treatment of the invention is simple, the external standard method is used for quantification, and the accuracy is poor

For another example, Northwest Pharmaceutical Journal, Volume 34, Issue 6, November 2019, is entitled "Simultaneous Determination of the Mass Concentrations of Fluoxetine, Norfluoxetine, Paroxetine and Fluvoxamine in Human Plasma by LC-MS / MS Method" ", the invention uses 200 μL plasma for protein precipitation, and the sample volume is large; using roxithromycin as the internal standard of all analytes lacks universal applicability and accuracy, and there may be matrix effect interference; the linear range of the four drugs All the same, not clinically proven, lack of rationality

In addition, because clinical samples are extremely precious, the pre-treatment sample volume should be as small as possible, and the injection volume will seriously pollute the instrument and increase the maintenance cost of the instrument.

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