Method for detecting concentration of antidepressant drugs in serum by using ultra-high performance liquid chromatography-tandem mass spectrometry technology

A high-performance liquid chromatography and ultra-high-performance liquid chromatography technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long analysis time, poor accuracy, and large sample consumption, and achieve simple processing, reliable detection methods, The effect of small sample size

CN111812222AInactive Publication Date: 2020-10-23南京品生医学检验实验室有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2020-10-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for detecting the concentration of antidepressant drugs in serum by an ultra-high performance liquid chromatography-tandem mass spectrometry technology. The antidepressant drugs comprise bupropion, agomelatine, hydroxybupropion, nortriptyline, o-desmethylvenlafaxine, mianserin, mirtazapine, venlafaxine, amitriptyline, doxepin, norfluoxetine hydrochloride, duloxetine, fluoxetine, fluvoxamine, citalopram, paroxetine, trazodone and vortioxetine. After a serum sample is pretreated, a to-be-detected substance is separated from a serum matrix by utilizing ultra-highperformance liquid chromatography, a calibration curve is established by utilizing a mass spectrum isotope internal standard quantitative method and taking a concentration ratio of the standard substance to an internal standard substance as an X axis and a peak area ratio of the standard substance to the internal standard substance as a Y axis, and the content of the drugs in serum is calculated.The method is high in sensitivity, high in specificity, accurate and simple in pretreatment process, separation and detection can be completed within 4.5 min, and the accuracy degree and precision basically meet the requirements.
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Description

technical field

[0001] The invention belongs to the technical field of blood detection, and in particular relates to a method for detecting the concentration of antidepressant drugs in serum by ultra-high performance liquid chromatography tandem mass spectrometry. Background technique

[0002] At present, the commonly used drugs for the treatment of depression are divided according to their mechanism of action: norepinephrine reuptake inhibitors, serotonin (5-HT) reuptake inhibitors (SSRIs), 5-HT and norepinephrine reuptake inhibitors SNRIs, norepinephrine and specific serotonergic antidepressants (NaSSAs), 5-HT2 receptor antagonists and 5-HT reuptake inhibitors, norepinephrine and dopamine reuptake inhibitors and melatonin receptor agonists.

[0003] The methods for determining antidepressants in human serum or plasma mainly include HPLC-UV method, GC-MS method and LC-MS / MS method in domestic and foreign literature reports, such as the literature "Quantification of 33antid...

Examples

Embodiment 1

[0050] 1. Materials

[0051] The samples of the methodological research experiment came from the serum samples collected from the outpatient clinic of Nanjing Drum Tower Hospital in October 2019.

[0052] (1) Instruments: Xevo TQ-S triple quadrupole mass spectrometer (Waters Corporation); UPLC I-Class ultra-high performance liquid chromatography system (with automatic sampler, Waters Corporation); SCILOGEX D2012 high-speed desktop centrifuge (USA) ; ultrapure water instrument (ELGA LabWater, UK); multi-tube vortex mixer (Vortex genie2, US); adjustable pipette (Eppendorf 0.5-10 μL, 10-100 μL, 100-1000 μL); glassware, graduated cylinder, etc. .

[0053] (2) Reagent consumables: MS grade methanol (Fisher, USA); HPLC grade methanol (Honeywell, USA); MS grade acetonitrile (Fisher, USA); HPLC grade acetonitrile (Honeywell, USA); MS grade formic acid (Fisher, USA) ; Chromatographic column: Phenomenex Kintex C18 (2.1×100 mm, 2.6 μm) (Phenomenex).

[0054] (3) Standard product: The ...