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Natural compound anti-aeromonas-hydrophila agent, natural feed and preparation method thereof

A natural technology of Aeromonas hydrophila, applied in antibacterial drugs, animal feed, animal feed, etc., can solve the problem of limited utilization of leftovers

Pending Publication Date: 2020-10-27
NEIJIANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of non-authentic medicinal materials and processed Chinese herbal medicine waste is very limited

Method used

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  • Natural compound anti-aeromonas-hydrophila agent, natural feed and preparation method thereof
  • Natural compound anti-aeromonas-hydrophila agent, natural feed and preparation method thereof
  • Natural compound anti-aeromonas-hydrophila agent, natural feed and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: Preparation of natural product anti-fish Aeromonas hydrophila infection feed

[0058] 1) Add the 6 kinds of extracts to the oil at the concentration of 1g / kg (referring to add one kind of extract per kg of basic feed) respectively in the following table 1, mix them evenly and add them to the basic feed to make the corresponding 8 kinds of extracts The feed pellets are identified as A0, A1, A2, A3, A4, A5, A6, A7, respectively.

[0059] Table 1

[0060] Additives A0 A1 A2 A3 A4 A5 A6 A7 Pomegranate Peel Ethanol Extract × × Turmeric Ethanol Extract × × Eucommia Ethanol Extract × × Ethanol Extract of Myrobalan × × Plum Ethanol Extract × × Scutellaria baicalensis ethanol extract × ×

[0061] in FIG. 1:

[0062] A0 refers to the basal feed without any extract added. In the following examples, A0 takes two copies, which are r...

Embodiment 2

[0093] The purchased crucian carp fry were raised in the breeding room for one week, and 405 crucian carp fry with an equivalent weight were selected and randomly divided into 9 treatment groups, each with 3 repeated fish tanks. The shape, size and color of each fish tank are the same, the size is 30×30×40cm and a built-in 800L / h flow aerator. The water in the fish tank takes potable tap water as the water source, and the water volume in the tank is 30L. The temperature of the breeding room was controlled at 22±1°C, and the aerator continued to supply oxygen. Give 9 treatment groups and feed respectively in embodiment 1 marked as " A0 (1) ", " A0 (2) ", " A1 ", " A2 ", " A3 ", " A4 ", " A5 ", " A6 " and "A7" feed, fed 4 times a day. After 7 days of feeding, the feeding was stopped for 1 day and the challenge experiment was carried out.

[0094] Intraperitoneal injection was used in the challenge experiment. The number of crucian carp in each tank is 15. Fish fed with "A0(1)...

Embodiment 3

[0102] The purchased crucian carp fry were raised in the breeding room for one week, and 405 crucian carp fry with an equivalent weight were selected and randomly divided into 9 treatment groups, each with 3 repeated fish tanks. The shape, size and color of each fish tank are the same, the size is 30×30×40cm and a built-in 800L / h flow aerator. The water in the fish tank takes potable tap water as the water source, and the water volume in the tank is 30L. The temperature of the breeding room was controlled at 22±1°C, and the aerator continued to supply oxygen. Give 9 treatment groups and feed respectively in embodiment 1 marked as " B0 (1) ", " B0 (2) ", " B1 ", " B2 ", " B3 ", " B4 ", " B5 ", " B6 " and "B7" feed, fed 4 times a day. After 7 days of feeding, the feeding was stopped for 1 day and the challenge experiment was carried out.

[0103] Intraperitoneal injection was used in the challenge experiment. The number of crucian carp in each tank is 15. The fish fed with ...

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Abstract

The invention discloses a natural compound anti-aeromonas-hydrophila agent, natural feed capable of resisting aeromonas hydrophila infection and a preparation method thereof. The compound anti-aeromonas-hydrophila agent consists of three natural medicinal plant extracts or above. The compound anti-aeromonas-hydrophila agent can improve the capability of fishes for resisting the aeromonas hydrophila infection during cultivation, can improve a survival rate of fishes during cultivation, does not have residues, does not have a toxic or side effect and does not pollute the environment.

Description

technical field [0001] The invention relates to a natural compound anti-Aeromonas hydrophila agent for fish, a natural product anti-Aeromonas hydrophila infection feed for fish and a preparation method of a natural product anti-Aeromonas hydrophila infection feed for fish. Background technique [0002] Aeromonas hydrophila is a Gram-negative short bacillus belonging to the genus Aeromonas in the Vibrio family. Aeromonas hydrophila is widely distributed in nature, commonly found in fresh water, sewage, sludge and soil. Cold-blooded animals such as fish and frogs are the natural hosts of this bacterium, which are pathogenic to a variety of fish, amphibians and reptiles, and are the main pathogenic bacteria that cause diseases of freshwater farmed animals. Aeromonas hydrophila can cause red fin disease of eel, vertical scale disease of carp and goldfish, printing disease of chain carp and bighead carp, fulminant hemorrhagic disease of silver carp, bacterial enteritis of herrin...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/30A61K36/9066A61P31/04
CPCA23K50/80A23K10/30A61K36/185A61K36/9066A61K36/539A61K9/0095A61P31/04A61K36/736Y02A40/818
Inventor 李华涛苟丹吴敏袁佳贺扬敬晓琴刘思妙袁萍龙娇董婷婷冯敏梁琪
Owner NEIJIANG NORMAL UNIV
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