Catecholamine substance magnetic molecularly imprinted polymer and preparation method and application thereof

Abstract

The invention discloses a catecholamine substance magnetic molecularly imprinted polymer and a preparation method thereof. Fe3O4 is used as a carrier, and template molecules (methoxyepinephrine and dopamine are used as double template molecules), alpha-methacrylic acid and ethylene glycol dimethacrylate form a magnetic molecularly imprinted polymer under the action of azodiisobutyronitrile througha thermal free radical polymerization reaction. The preparation method comprises the following steps: taking magnetic Fe3O4 nanoparticles as a raw material; preparing vinylated Fe3O4 nanoparticles tomodify the surfaces of the vinylated Fe3O4 nanoparticles with carbon-carbon double bonds; preparing a magnetic molecularly imprinted polymer by adopting a surface molecular imprinting technology; theadsorbent has relatively strong selection capability and adsorbability on methoxyepinephrine, methoxynorepinephrine and 3-methoxytyramine molecules, has high adsorption capacity on a target to-be-detected object, can be recycled, and can be used for efficiently separating and enriching methoxyepinephrine, methoxynorepinephrine and 3-methoxytyramine molecules in a urine sample.

Description

technical field [0001] The invention belongs to the technical field of synthesis of molecularly imprinted polymers, and in particular relates to a magnetic molecularly imprinted polymer of catecholamines and its preparation method and application. Background technique [0002] Pheochromocytomas are neuroendocrine tumors that arise from the adrenal medulla or paraganglia. The clinical presentation varies widely, but the most common manifestations are headache, sweating, palpitations, and hypertension. Due to the excessive secretion of catecholamines, these tumors can cause fatal cardiovascular complications. Currently, methoxyepinephrine (MN), methoxynorepinephrine (NMN) and 3-methoxytyramine (3-MT) are clinically used as biochemical indicators for the diagnosis of pheochromocytoma. [0003] Molecularly imprinted polymer (MIP) is an artificial receptor material that can specifically bind target molecules. Due to its high stability, simple synthesis method and low cost, it ca...

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Problems solved by technology

[0004]Preparation of a Molecularly Imprinted Polymer Nanotubes Membrane and ItsApplication in the Determination of Catecholamines in Urine Samples [Journal Papers] Qiu Xiuzhen, Hua Yongbiao, Guo Huishi, Lu Wenguan, QIU Xiuzhen, HUA Yongbiao, GUO Huishi, LU Wenguan - In the journal paper of "Journal of Chemistry of Universities" 2018 4 issue, dopamine ( DA) as a template and porous anodized aluminum oxide membrane (AAO) as a reaction carrier, a dopamine molecularly imprinted polymer nanotube membrane (AAO@MIP) was synthesized to detect the content of catecholamines in human urine. The disadvantages are: AAO@MIP has low repeatability, low recovery rate, low adsorption performance, low selectivity and adsorption for dopamine, epinephrine and norepinephrine, etc.

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