Preparation method and application of pilose antler stem cell exosome for resisting skin aging

An anti-aging, stem cell technology, applied in cell dissociation methods, preparations for skin care, epidermal cells/skin cells, etc., can solve problems such as stimulation of epithelial-mesenchymal cell transformation, immune rejection, and promotion of tumor cell metastasis

Pending Publication Date: 2020-10-27
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the direct use of stem cells has disadvantages in terms of storage and transportation inconvenience and immune rejection
Moreover, stem cells are easy

Method used

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  • Preparation method and application of pilose antler stem cell exosome for resisting skin aging
  • Preparation method and application of pilose antler stem cell exosome for resisting skin aging

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0030] Example 1:

[0031] 1. Isolation and culture of deer antler stem cells; deer antler stem cells are derived from deer antler area periosteum tissue, antler stalk periosteum tissue or deer antler mesenchymal tissue.

[0032] (1) The specific operation process of collecting the periosteum tissue in the deer antler area is as follows: anesthetize the 1-year-old male deer that has not started to develop in the deer antler area. Next, cut open the skin of the velvet area, expose the periosteum of the velvet area, clean up the loose connective tissue, and then use a clean scalpel to cut along the velvet area (the lowest part of the extra ridge), and then use mouse-tooth forceps to cut the cut periosteum Tear off from the bone and quickly put it into the transport liquid, which is DMEM medium containing double double antibody.

[0033] (2) The specific operation process of collecting staghorn stalk periosteal tissue is: anesthetize the 2-year-old male deer, prepare the skin of the ho...

Example Embodiment

[0045] Example 2:

[0046] (1) The velvet stem cell exosomes obtained in Example 1 were tested for their DPPH free radical scavenging activity.

[0047] Weigh 1 mg of DPPH and dissolve it in 20 ml of absolute ethanol, add 1 ml of DPPH solution and 0.01 ml of velvet stem cell exosomes to each tube of reaction system, mix well, and measure the absorbance at 519 nm after standing for 30 minutes.

[0048] A blank medium was used as a blank control, and the sample control group consisted of 1 mL ethanol solution and 0.01 mL velvet stem cell exosomes. Calculate the DPPH free radical scavenging rate according to the following formula.

[0049] DPPH free radical scavenging rate=[1-(A sample group-A sample control group) / A blank control group]X100%

[0050] It is determined that the conditioned medium of deer antler stem cells and its freeze-dried powder have an obvious effect of scavenging DPPH free radicals, and the scavenging rate can reach APC-EXO: 77.4%, PPC-EXO: 75.3%, and RMC-EXO: 79.53%...

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Abstract

The invention discloses a preparation method and application of a pilose antler stem cell exosome for resisting skin aging, and belongs to the technical field of tissue engineering. In order to solvethe problem of skin aging resistance, the invention provides an exosome which is the pilose antler stem cell exosome. The antler stem cell exosome contains a large amount of various components such ascytokines, growth factors, miRNA and mRNAs and is easily fused with cell membranes of adjacent cells, bioactive substances are selectively delivered to recipient cells, information transmission is performed among different cells, and signal transmission among the cells is regulated. Compared with human bone marrow mesenchymal stem cells, the antler stem cell exosome obtained by the method has theadvantages that pilose antler stem cells are easy to obtain, the proliferation speed is higher than that of the human bone marrow mesenchymal stem cells by more than ten times, DPPH free radicals canbe removed, and the antler stem cell exosome has the anti-oxidation function and has the obvious promotion effect on the proliferation of Hacat cells and NHDF cells.

Description

technical field [0001] The invention relates to a preparation method and application of antler stem cell exosomes for anti-aging skin, and belongs to the technical field of tissue engineering. Background technique [0002] Antler is the only known mammalian organ that can fully regenerate, and its growth rate can reach 2cm / d. The complete regeneration and rapid growth ability of deer antler is mainly due to the presence of deer antler stem cells. It has been identified that antler stem cells are a special stem cell type between embryonic stem cells and mesenchymal stem cells. Compared with bone marrow mesenchymal stem cells, umbilical cord mesenchymal stem cells and other stem cells commonly used at present, antler stem cells are easier to obtain, proliferate faster, and secrete a large amount of cytokines and biologically active factors. [0003] In recent years, the use of stem cell transplantation to supplement human stem cells for anti-aging is considered to be a new a...

Claims

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Application Information

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IPC IPC(8): C12N5/071A61K8/98A61Q19/08
CPCC12N5/063A61K8/985A61Q19/08C12N2509/00C12N2509/10A61K2800/805
Inventor 孙红梅赵海平王大涛刘振郭倩倩
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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