Exosome preparation deriving from human umbilical cord mesenchymal stem cells

Abstract

The invention discloses an exosome preparation deriving from human umbilical cord mesenchymal stem cells. The exosome preparation contains the following raw materials: a Lonza UltraCULTURE serum-freemedium, a PALL UltroserG serum substitute, L-Glutamine and ganoderan. The exosome preparation is prepared by the following steps: dissociating cultured cells, washing the cells by virtue of a sodium chloride injection, and adding the cells into culture supernatant; putting culture supernatant into an ultra-low-temperature refrigerator for refrigeration; taking out the culture supernatant for unfreezing, carrying out high-speed centrifugation, concentrating the supernatant by virtue of an ultrafiltration membrane, and adding sodium hyaluronate, human serum albumin and trehalose, so as to prepare the exosome preparation deriving from the human umbilical cord mesenchymal stem cells. By utilizing a serum-free culture system in a preparation method of the exosome preparation deriving from the human umbilical cord mesenchymal stem cells, the quality is stable, the batch difference is small, and an exogenous pathogenic factor is not introduced; by adding the ganoderan, proliferation of the umbilical cord mesenchymal stem cells can be promoted, and secretion of exosomes by the umbilical cord mesenchymal stem cells can be promoted; a complete culture medium and a condition culture medium are used for acquiring the exosomes, so that the yield is increased; and after the cells are frozen and dissociated, contents are mixed into the culture supernatant, so that the concentration of activesubstances is increased, and the exosome preparation is a face-beautifying anti-aging biological preparation with an excellent effect.

Description

technical field [0001] The invention relates to the technical field of mesenchymal stem cells, in particular to a method for preparing an exosome preparation derived from human umbilical cord mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells are a kind of pluripotent stem cells, which have all the common characteristics of stem cells, that is, self-renewal and multidirectional differentiation capabilities. Major breakthroughs have been made in vascular diseases, liver cirrhosis, nervous system diseases, partial knee meniscectomy injury repair, autoimmune diseases, etc., saving the lives of more patients. Due to the large size of human umbilical cord mesenchymal stem cells, there is a blood-air barrier barrier in human application, and the number that can reach the action site is small, and maintaining cell activity during storage and transportation requires cumbersome processes and a lot of cost. Exosomes are subcellular double-layer membrane vesi...

AI Technical Summary

Problems solved by technology

Due to the large size of human umbilical cord mesenchymal stem cells, there is a blood-gas barrier barrier in human application, and the number that can reach the action site is small, and maintaining cell activity during storage and transportation requires cumbersome processes and a lot of cost.

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