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System for in vitro detection of digestive tract five cancers, composition used in the system, and kit comprising the composition

A technology for in vitro detection and digestive tract, applied in the direction of microbial determination/testing, biochemical equipment and methods, etc., can solve the problem of low accuracy

Pending Publication Date: 2020-10-27
BIOCHAIN BEIJING SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0015] Based on this, aiming at the low accuracy of screening and early screening of patients with five digestive tract cancers (esophageal cancer, gastric cancer, colorectal cancer, liver cancer, pancreatic cancer) existing in the prior art, the present application provides a method for A system for detecting five cancers of the digestive tract. The system provided by this application can improve the accuracy of early screening of five cancers of the digestive tract. This application also provides a composition for detecting five cancers of the digestive tract in this system. This application provides The composition can accurately detect five cancers of the digestive tract, and the application also provides a kit comprising the composition

Method used

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  • System for in vitro detection of digestive tract five cancers, composition used in the system, and kit comprising the composition
  • System for in vitro detection of digestive tract five cancers, composition used in the system, and kit comprising the composition
  • System for in vitro detection of digestive tract five cancers, composition used in the system, and kit comprising the composition

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0504] Embodiment 1: questionnaire survey

[0505] According to "Guidelines for Screening and Endoscopic Diagnosis and Treatment of Early Colorectal Cancer in China (2014, Beijing)", "Consensus Opinions on Screening and Endoscopic Diagnosis and Treatment of Early Gastric Cancer in China (2014, Changsha)", "Screening and Endoscopy for Early Esophageal Cancer in China" According to the guidelines of Diagnosis and Treatment Expert Consensus (2014), "Primary Liver Cancer Diagnosis and Treatment Guidelines (2017 Edition)", "Pancreatic Cancer Comprehensive Diagnosis and Treatment Guidelines (2018 Edition)", a comprehensive questionnaire for the five digestive tract cancers was established ( figure 1 As shown), the interpretation method of the questionnaire survey is carried out using the contents of the following table:

[0506] 1. Risk factors for colorectal cancer

[0507]

[0508] 2. Risk factors for gastric cancer

[0509]

[0510]

[0511] 3. Risk factors for esophag...

Embodiment 2

[0518] Embodiment 2: the detection of marker

[0519] 10 mL of peripheral blood was drawn from the subject to be tested, and 3.5 mL of plasma was obtained by separation. Genomic DNA of the sample is then extracted, and the genomic DNA sample is pretreated such that the unmethylated cytosine base at the 5' position is converted to uracil, thymine, or another base not available for cytosine in hybridization behavior. one base. In this example, this pretreatment was achieved by bisulfite reagent treatment. The extraction and processing of the DNA can be carried out by any standard means in the prior art. Specifically, in this embodiment, all sample DNA extraction and bisulfite DNA modification are carried out by using Boercheng Company Extracted from the plasma processing kit. Then, the above-mentioned primer-probe composition of MT1A, primer-probe composition of EPO, Septin9 gene primer-probe composition, primer-probe composition of RNF180, and primer-probe composition of B...

Embodiment 3

[0525] Embodiment 3: comprehensive evaluation of digestive tract five cancers

[0526] In order to verify the accuracy and practicability of the comprehensive evaluation system for the five digestive tract cancers, large-scale screening is being adopted. Since the incidence of cancer is relatively low compared to the total population, it is necessary to conduct reliable conclusions to draw reliable conclusions. At least tens of thousands of cases of research. Therefore, we entrusted a third-party hospital unit to conduct a blind test, and selected 100 samples of people to be tested as the sample bank. A certain number of colorectal cancer samples, gastric cancer samples, esophageal cancer samples, liver cancer samples, pancreas cancer samples, and pancreatic cancer samples were randomly mixed into the sample library. Cancer samples and normal human samples.

[0527] First, the comprehensive evaluation system is used to conduct a questionnaire on the 100 cases of persons to ...

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Abstract

The invention provides a system for detecting five cancers of the digestive tract in vitro. The system comprises a composition of nucleic acid for detecting the methylation state of a target gene, a kit containing the composition, a conventional detection reagent containing other conventional tumor markers and cancer high-risk factor markers, and a questionnaire. According to the system, based onthe detection result of the kit, a detected person can be scientifically and comprehensively evaluated, and further follow-up or definite diagnosis suggestions are given. By using the system for detecting the five cancers of the digestive tract in vitro, the five cancers (esophagus cancer, gastric cancer, colorectal cancer, liver cancer and pancreatic cancer) of the digestive tract can be accurately and rapidly detected at a time, and the system can be used for screening and early screening of the five cancers of the digestive tract.

Description

technical field [0001] This application belongs to the field of biotechnology, and relates to a system for in vitro detection of five cancers of the digestive tract (esophageal cancer, gastric cancer, colorectal cancer, liver cancer, and pancreatic cancer), specifically, a system for in vitro detection of five cancers of the digestive tract Compositions including nucleic acids for detecting the methylation status of target genes, and kits containing the compositions, which also include conventional detection reagents containing other conventional tumor markers and cancer risk factor markers, and questionnaires. Using the above system to conduct a comprehensive evaluation to assess the risk of five digestive tract cancers for accurate, rapid and early screening of digestive tract cancers. Background technique [0002] Cancer is a disease that seriously threatens public health. The latest data on the global cancer situation (GLOBOCAN2012) released by the International Agency...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/154
Inventor 韩晓亮周光朋吴振王建铭
Owner BIOCHAIN BEIJING SCI & TECH