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Full-automatic real-time fluorescent quantitative PCR detection system

A real-time fluorescent quantitative and detection system technology, applied in the field of genetic and life science instruments, which can solve problems such as hidden safety hazards, time-consuming, and biological hazards for operators

Pending Publication Date: 2020-10-30
上海基灵生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current nucleic acid detection process has a low degree of automation and requires the training of professional skills for operators, which poses a certain biological hazard to operators, especially some high-risk samples, which take a lot of time and have certain safety hazards

Method used

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  • Full-automatic real-time fluorescent quantitative PCR detection system
  • Full-automatic real-time fluorescent quantitative PCR detection system
  • Full-automatic real-time fluorescent quantitative PCR detection system

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Embodiment Construction

[0025] In order to make the above objects, features and advantages of the present invention more comprehensible, specific implementations of the present invention will be described in detail below in conjunction with the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. However, the present invention can be implemented in many other ways different from those described here, and those skilled in the art can make similar improvements without departing from the connotation of the present invention, so the present invention is not limited by the specific implementations disclosed below.

[0026] It should be noted that when an element is referred to as being “fixed” to another element, it can be directly on the other element or there can also be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected ...

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Abstract

The invention discloses a full-automatic real-time fluorescent quantitative PCR detection system. The system comprises a detection main body, the detection main body is composed of a shell assembly and an internal assembly, the shell assembly is composed of a shell, a screen and a bin gate, and the internal assembly is composed of an electric control assembly, a magnetic stirring assembly, a kit loading and unloading assembly, a core module, an XY-axis moving assembly, a pipetting assembly, a cap screwing assembly, an air duct and an optical assembly; wherein the software architecture of a detection main body is divided into a UI layer, a data control layer and a data link layer, the data control layer is divided into a data management layer, a logic control layer and an algorithm library,and the data link layer comprises a configuration file, a database, a communication protocol and a physical interface. The system is reasonable in structure, integrates an automatic nucleic acid extraction function and an automatic PCR amplification function, and realizes an automatic detection process.

Description

technical field [0001] The invention relates to the technical field of genes and life science instruments, in particular to a fully automatic real-time fluorescent quantitative PCR detection system. Background technique [0002] Nucleic acid detection is aimed at viral DNA and RNA, and the detection of viruses in clinical diseases such as hepatitis B, hepatitis C, and AIDS. It mainly detects whether there is viral nucleic acid in the blood, as a basis for diagnosing whether the body has pathogen infection. [0003] The current nucleic acid detection process has a low degree of automation and requires the training of professional skills for operators, which poses a certain biological hazard to operators, especially for some high-risk samples, which takes a lot of time and also poses certain safety hazards. Contents of the invention [0004] The purpose of the present invention is to solve the shortcomings in the prior art, and propose a fully automatic real-time fluorescent...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/00
CPCC12Q1/6851B01L7/52C12Q2531/113C12Q2563/107C12Q2561/113
Inventor 李杰黄挺朱旭崔立奇
Owner 上海基灵生物科技有限公司
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