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Novel coronavirus IgA/IgG antibody joint inspection immunochromatographic test strip

An immunochromatographic test strip, coronavirus technology, applied in the biological field, can solve problems such as the decline of antibody levels, and achieve good specificity, low cost, and short detection time

Pending Publication Date: 2020-11-03
NANTONG EGENS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that IgA is particularly prominent in critically ill patients, but over time, antibody levels decline and IgG remains positive

Method used

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  • Novel coronavirus IgA/IgG antibody joint inspection immunochromatographic test strip
  • Novel coronavirus IgA/IgG antibody joint inspection immunochromatographic test strip
  • Novel coronavirus IgA/IgG antibody joint inspection immunochromatographic test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A novel coronavirus IgA / IgG antibody joint detection immunochromatographic test strip, such as figure 1 As shown, on the substrate 1, a sample pad 2, a colloidal gold adsorption pad 3, an antibody carrying membrane 4 and a water-absorbing pad 5 are sequentially overlapped;

[0037] The colloidal gold adsorption pad 3 is coated with colloidal gold-labeled novel coronavirus recombinant antigen and colloidal gold-labeled mouse IgG; the sequence of the novel coronavirus recombinant antigen is shown in SEQ ID NO.1;

[0038] The antibody carrying membrane 4 is provided with a detection line T1, a detection line T2 and a quality control line C at intervals, the detection line is set on the side close to the colloidal gold adsorption pad 3, and the quality control line C is set on the side close to the water absorption pad 5. One side: the detection line T1 is coated with anti-human IgA monoclonal antibody, the detection line T2 is coated with anti-human IgG monoclonal antibody...

Embodiment 2

[0041] This embodiment provides a method for preparing the novel coronavirus IgA / IgG antibody joint detection immunochromatographic test strip in embodiment 1, comprising the following steps:

[0042] 1. Preparation of antibody-carrying membrane:

[0043] (1) Select a nitrocellulose membrane with a pore size of 3 μm to 10 μm, and cut the membrane into specifications with a width of 2.0 cm and a length of 30.5 cm as required, for later use.

[0044] (2) Use 0.1M Tris-HCL buffer solution with pH 8.0 to prepare anti-human IgG monoclonal antibody for detection line coating. mL; anti-human IgA monoclonal antibody, antibody concentration is (0.3~0.5) mg / mL, is 0.3mg / mL in the present embodiment, is prepared with the sodium chloride buffer solution of mass percentage content 0.85% for quality control line bag The goat anti-mouse IgG polyclonal antibody used had an antibody concentration of (1.5 to 1.8) mg / mL, 1.5 mg / mL in this example.

[0045](3) Select the antibody-coated surface...

Embodiment 3

[0059] This embodiment provides a new coronavirus IgA / IgG antibody joint detection immunochromatographic reagent card, including the new coronavirus IgA / IgG antibody joint detection immunochromatographic test strip in Example 1.

[0060] Further, such as figure 2 As shown, it also includes a cartridge 7, and the novel coronavirus IgA / IgG antibody joint detection immunochromatographic test strip in Example 1 is installed inside the cartridge.

[0061] The clamping case 7 includes an upper cover 8 and a lower cover;

[0062] The upper cover 8 is provided with a sampling hole 9 and a viewing window 10;

[0063] The sample loading hole 9 corresponds to the sample pad 2 of the novel coronavirus IgA / IgG antibody joint detection immunochromatography test strip;

[0064] The window 10 corresponds to the antibody-carrying membrane 4 of the new coronavirus IgA / IgG antibody joint detection immunochromatography test strip.

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Abstract

The invention provides a novel coronavirus IgA / IgG antibody joint inspection immunochromatographic test strip. The novel coronavirus IgA / IgG antibody joint inspection immunochromatographic test stripcomprises a colloidal gold adsorption pad and an antibody bearing film, the colloidal gold adsorption pad is coated with a colloidal gold labeled novel coronavirus recombinant antigen and a colloidalgold labeled mouse IgG; a detection line T1, a detection line T2 and a quality control line C are arranged on the antibody bearing membrane at intervals, and the detection line T1 is coated with an anti-human IgA antibody; and the detection line T2 is coated with an anti-human IgG antibody. The immunochromatographic test strip can simultaneously detect novel coronavirus IgA and IgG antibodies, hasthe advantages of short detection time of only 10 min, low cost, easy operation and low technical requirements, is convenient for on-site collection of samples (especially oral mucosa exudates), andis suitable for on-site, instant and rapid detection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a novel coronavirus IgA / IgG antibody detection kit. Background technique [0002] Coronaviruses are enveloped RNA viruses that are widely distributed in humans, other mammals, and birds and can cause respiratory, intestinal, hepatic, and neurological diseases. Seven coronaviruses are currently known to cause disease in humans. Four viruses—229E, OC43, NL63, and HKU1—are ubiquitous and cause common cold symptoms in immunocompetent individuals. Three other strains—severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), and 2019 novel coronavirus (COVID-19)—are zoonotic, Sometimes fatal diseases are associated. IgG and IgA antibodies can be detected 2-3 weeks after exposure to the novel coronavirus. Studies have shown that IgA is particularly prominent in critically ill patients, but over time, antibody levels decline and Ig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/58G01N33/558
CPCG01N33/6854G01N33/56983G01N33/587G01N33/558G01N2333/165G01N2469/20
Inventor 欧卫军孙一品张娟顾飞陈兰兰
Owner NANTONG EGENS BIOTECH
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