Hair growing oil and preparation method thereof
A growth factor and oily liquid technology, applied in the field of hair tonic and its preparation, can solve the problems of difficult biological activity, poor stability, and difficult hair growth effect
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[0033] The present invention also provides the preparation method of the hair growth oil described in the above technical solution, comprising the following steps:
[0034] (1) geranic acid and choline are mixed to carry out metathesis reaction to obtain ionic liquid;
[0035] (2) drying the ionic liquid obtained in the step (1) to obtain an oily liquid;
[0036] (3) mixing keratinocyte growth factor and PBS buffer to obtain growth factor solution;
[0037] (4) mixing the oily liquid obtained in the step (2) with the growth factor solution obtained in the step (3) to obtain hair growth oil;
[0038] The steps (1)-(2) and step (3) are not in order.
[0039] In the present invention, geranic acid and choline are mixed to carry out metathesis reaction to obtain ionic liquid. In the present invention, the mixing of geranic acid and choline is preferably carried out under stirring conditions. The present invention does not have a special limitation on the stirring time, as long...
Embodiment 1
[0047] (1) get 48.195wt% geranic acid (purity of 90%) and 29.076wt% choline in a round-bottomed flask and stir at room temperature until no carbon dioxide gas is generated to obtain an ionic liquid;
[0048] (2) vacuum drying the ionic liquid obtained in step (1) at 60° C. for 48h to obtain an oily liquid;
[0049] (3) Dissolving 0.005wt% KGF-2 in 22.724wt% PBS buffer to obtain a growth factor solution;
[0050] (4) uniformly mixing the oily liquid obtained in the step (2) and the growth factor solution obtained in the step (3) to obtain a hair growth oil.
Embodiment 2
[0052] (1) get 48.197wt% geranic acid (purity of 90%) and 29.077wt% choline in a round-bottomed flask and stir at room temperature until no carbon dioxide gas is generated to obtain an ionic liquid;
[0053] (2) vacuum drying the ionic liquid obtained in step (1) at 60° C. for 48h to obtain an oily liquid;
[0054] (3) Dissolving 0.001wt% KGF-2 in 22.725wt% PBS buffer to obtain a growth factor solution;
[0055] (4) uniformly mixing the oily liquid obtained in the step (2) and the growth factor solution obtained in the step (3) to obtain a hair growth oil.
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