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Erythrocin degradation bacterium pseudomonas aeruginosa RJJ-1 and application thereof

A technology of Pseudomonas aeruginosa and erythromycin, which is applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems that erythromycin cannot be degraded, microorganisms cannot survive, etc. Achieve excellent social benefits and strong degradation ability

Active Publication Date: 2020-11-06
CHANGZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And well-known in the art, methanol is a kind of poisonous organic solvent, can change the permeability of cell membrane, and microorganism can't survive in the methanol solution of 96% (v / v), more unlikely to degrade erythromycin therein
Therefore, there is no reliable report about Pseudomonas aeruginosa being used to degrade erythromycin at present, and there is no single use of Pseudomonas aeruginosa (Pseudomonas aeruginosa as the only active bacterial species) for degrading erythromycin to report

Method used

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  • Erythrocin degradation bacterium pseudomonas aeruginosa RJJ-1 and application thereof
  • Erythrocin degradation bacterium pseudomonas aeruginosa RJJ-1 and application thereof
  • Erythrocin degradation bacterium pseudomonas aeruginosa RJJ-1 and application thereof

Examples

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Effect test

Embodiment 1

[0025] Example 1 Screening and identification of Pseudomonas aeruginosa RJJ-1 of the present invention

[0026] 1. Screening

[0027] Weigh a certain amount of bacterial residue, add distilled water, and dilute 10 times. Take 1 mL of the supernatant, add 9 mL of sterile water for gradient dilution, and dilute to 1000 times, take the supernatant and add MSM sterile medium containing erythromycin, and place it in a shaker at 37 ° C and 180 r / min for 5 days. Spread the supernatant obtained above evenly on the MSM solid medium containing erythromycin, then put it into a 37°C incubator and culture it for 3-5 days, and separate it by streaking on an LB plate, tentatively named "erythromycin degradation Bacteria RJJ-1".

[0028] 2. Strain identification

[0029] The genomic DNA of erythromycin-degrading bacteria RJJ-1 was obtained by shaking and crushing method, and the 16S rRNA gene of the strain was amplified by PCR method and sent to Shanghai Jierui Biological Company for seque...

experiment example 1

[0054] Degradation experiment of erythromycin in experimental example 1 medium

[0055] The Pseudomonas aeruginosa RJJ-1 of the present invention is cultured in LB liquid medium, the thalline is collected by centrifugation, resuspended with sterile water, and diluted to OD 600 =1 suspension, take 1mL of the aforementioned suspension and inoculate it into 100.0mL of basic liquid inorganic salt medium containing 100mg of erythromycin, place it in a constant temperature shaker at 180r / min, and cultivate it at 37°C for 72h. The degradation rate is 68.2%. The effect of mymycin degradation efficiency is obvious ( figure 2 ).

[0056] The results of this experimental example show that Pseudomonas aeruginosa RJJ-1 can effectively degrade erythromycin.

experiment example 2

[0057] Degradation experiment of erythromycin in experimental example 2 soil

[0058] The Pseudomonas aeruginosa RJJ-1 of the present invention is cultured in LB liquid medium, the thalline is collected by centrifugation, resuspended with sterile water, and diluted to OD 600 =1 suspension, get 1mL of the aforementioned suspension and inoculate it into 100 grams of sterilized soil, and add 100mg of erythromycin, and cultivate it for 72h at 37°C. After testing, the degradation rate of erythromycin is: 46.22% ( image 3 ).

[0059] The results of this experimental example show that Pseudomonas aeruginosa RJJ-1 of the present invention can effectively degrade erythromycin in soil, and can be used for the treatment of erythromycin pollution in soil.

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Abstract

The invention discloses pseudomonas aeruginosa and application thereof, and belongs to the field of microbial environmental restoration. The pseudomonas aeruginosa disclosed by the invention is preserved in China General Microbiological Culture Collection Center (CGMCC), has the preservation number of CGMCC NO.19234, has very high degradation ability for erythrocin, and has good prospect when being applied to harmless treatment of erythrocin zymophyte residues and restoration of water and soil.

Description

technical field [0001] The invention belongs to the field of microbial environment restoration. Background technique [0002] With the increasing demand for erythromycin, erythromycin pharmaceutical factories have produced more and more seriously polluted fungus residues during the production process, the main components of which are the mycelium produced by cultivating erythromycin Medium, metabolites produced during fermentation, erythromycin-degrading bacteria and a small amount of erythromycin, etc. [0003] The residual erythromycin in the fungus residue will enter the soil and water, affecting the growth and development of plants, animals and microorganisms, and the erythromycin accumulated in the animal body will also accumulate in the food chain and be ingested by the human body, causing allergic reactions in the human body , cause food poisoning in severe cases, and even bring carcinogenic, teratogenic, mutagenic and other hazards. On the other hand, food grown in...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34A62D3/02A62D101/26A62D101/28C02F101/34C02F101/38C12R1/385
CPCC12N1/20A62D3/02C02F3/34A62D2101/26A62D2101/28C02F2101/34C02F2101/38C12R2001/385C12N1/205
Inventor 沈云鹏任建军邓留杰王珍珠李志杰何松董丽萍梁硕罗成韩丽丽
Owner CHANGZHOU UNIV
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