Application of OXS2 gene and encoded protein thereof in improvement of salt stress resistance of plants
A transgenic plant, salt stress technology, applied in the application field of the OXS2 gene and its encoded protein in improving the salt stress resistance of plants, can solve the problems of blocked water absorption, water shortage in plants, changing the spatial structure of proteins, etc., and achieve enhanced resistance. The ability of salt, the effect of increasing yield
Active Publication Date: 2020-11-06
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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Problems solved by technology
High concentrations of salt create osmotic stress, which prevents plants from absorbing water from the soil, causing water shortages in plants
In addition to these visible hazards, salt stress can also adversely affect the physiological and biochemical processes of plants
Sodium ions and chloride ions will change the spatial structure of some proteins and affect their function
Certain biochemical reactions that require potassium ions to participate cannot proceed normally after being replaced by sodium ions
[0004] According to existing research, among the 500 million mu of saline-alkali soil, about 200 million mu of it has the potential for agricultural utilization. Therefore, if these saline-alkali soil can be used efficiently to grow food crops, the economic benefits and social Benefits are immeasurable
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[0034] In order to make the object, technical solution and technical effect of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific implementations described in this specification are only for explaining the present invention, not for limiting the present invention.
[0035] The experimental materials and reagents used, unless otherwise specified, are conventional consumables and reagents that can be obtained from commercial channels.
[0036] The molecular biology experiment techniques adopted in the following examples include PCR amplification, plasmid extraction, plasmid transformation,
[0037] DNA fragment ligation, enzyme digestion, gel electrophoresis, etc., unless otherwise specified, are usually operated according to conventional methods. For details, please refer to "Molecular Cloning Experiment Guide" (Third Edition) (Sambrook J, Russell DW, ...
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The invention discloses an application of an OXS2 gene and an encoded protein thereof in improvement of salt stress resistance of plants. The invention finds that when OXS2AT3 is massively expressed in arabidopsis thaliana, the salt resistance of arabidopsis thaliana can be specifically enhanced. Arabidopsis thaliana OXS2AT3 is found to improve the resistance to salt stress by up-regulating the expression quantity of the salt stress response gene, so that the gene segment has great potential to be applied to cultivation of transgenic crops with salt resistance, and the yield of grain crops isincreased. Compared with the traditional technology, only the C-end short fragment of the OXS2 is over-expressed, and the operation is simpler and easier.
Description
technical field [0001] The invention belongs to the field of molecular biology, and specifically relates to the application of OXS2 gene and its coded protein in improving the resistance of plants to salt stress. Background technique [0002] Saline-alkali soil refers to the conductivity of the saturated extract solution in the root zone at a temperature of 25 °C greater than 4dS m -1 (equivalent to 40mM NaCl) with a soil exchangeable sodium percentage of 15%. There are many causes of soil salinization, which can be divided into two categories: one is caused by environmental factors; the other is caused by human activities. Environmental factors include temperature, precipitation and soil moisture content. Soil salinization caused by environmental factors is mainly because the salt at the bottom of the soil is brought to the soil surface with the evaporation of water under high temperature conditions, and the continuous accumulation of these salts leads to soil salinizatio...
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Login to View More IPC IPC(8): C12N15/82C07K14/415C12N15/29A01H5/00A01H6/20
CPCC12N15/8273C07K14/415
Inventor 区永祥蔡佳佳李勇青
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI