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Bovine immunoglobulin (IgG) double-antibody sandwich colloidal gold immunochromatographic test strip, preparation method, kit and detection method

An immunochromatographic test strip and immunoglobulin technology, which is applied in the field of detection reagent strips and kits, can solve the problems of inability to achieve high-throughput processing and shorten the detection time, and achieve the effect of rapid and accurate screening and shortening of the detection time.

Inactive Publication Date: 2020-11-06
HANGZHOU BINGUO INFORMATION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to overcome the above-mentioned shortcomings in the field of bovine immunoglobulin (IgG) detection in the prior art, and the inadequacy of being unable to realize high-throughput processing, and provides a bovine immunoglobulin (IgG) double-antibody sandwich Colloidal gold immunochromatographic test strips, preparation methods, kits and detection methods can realize rapid quantitative detection of bovine immunoglobulin in samples, greatly shorten the detection time, and can realize rapid and accurate screening of large batches of samples check

Method used

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  • Bovine immunoglobulin (IgG) double-antibody sandwich colloidal gold immunochromatographic test strip, preparation method, kit and detection method
  • Bovine immunoglobulin (IgG) double-antibody sandwich colloidal gold immunochromatographic test strip, preparation method, kit and detection method
  • Bovine immunoglobulin (IgG) double-antibody sandwich colloidal gold immunochromatographic test strip, preparation method, kit and detection method

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Experimental program
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Embodiment 1

[0038] Such as figure 1 As shown, a bovine immunoglobulin (IgG) double-antibody sandwich colloidal gold immunochromatographic test strip provided in this embodiment includes a base plate, and an absorbent pad 1, a chromatographic membrane 2, and a binding pad that are overlapped on the PVC base plate. Material release pad 5 and sample pad 6, the bovine immunoglobulin antibody a complex labeled with colloidal gold is coated on the conjugate release pad, a detection line (TestLine, T line) and a quality control line (TestLine, T line) are provided on the chromatographic membrane Control Line, C line), the detection line is coated with anti-bovine immunoglobulin antibody b, and the quality control line is coated with goat anti-mouse IgG.

[0039]The detection line on the chromatographic membrane is coated with anti-bovine immunoglobulin antibody b at a concentration of 0.5-2 mg / mL. The quality control line on the chromatographic membrane is coated with goat anti-mouse IgG at a c...

Embodiment 2

[0046] The present application simultaneously provides a method for preparing a colloidal gold-labeled bovine immunoglobulin antibody a complex, the method comprising the following steps:

[0047] Take 10mL of the colloidal gold solution that has been fired, and use 0.1MK 2 CO 3 Adjust the pH of the solution to 7.5, add 1 mL of bovine immunoglobulin antibody solution with a concentration of 110 μg / mL dropwise under magnetic stirring, mix well, and let stand for 1 h;

[0048] Add 1.3mL borate buffer solution containing 10% BSA and 1% PEG dropwise to the solution after standing still, mix well, let stand for 1h, centrifuge at 13500r / min for 30min, discard the supernatant, reconstitute with borate buffer solution and repeat Centrifuge once, and finally settle the precipitate with 1% BSA and 5% sucrose to 1 mL, that is, the concentrated solution of colloidal gold-labeled bovine immunoglobulin antibody a complex. The colloidal gold is 20-50nm colloidal gold particles obtained by ...

Embodiment 3

[0050] The present embodiment provides a kind of preparation method of bovine immunoglobulin (IgG) double antibody sandwich colloidal gold immunochromatography test strip, and described method comprises the following steps:

[0051] Step 1, prepare the sample pad: the sample pad is made of glass fiber membrane, and the glass fiber membrane is treated with phosphate buffer solution containing 1% BSA, 2% sucrose, 0.1% PEG, and 0.05% Tween-20 for 1 hour, and then dried Make a sample pad;

[0052] Step 2, prepare the conjugate release pad: the conjugate release pad adopts glass fiber membrane, pretreat the glass fiber membrane with phosphate buffer saline containing 1% BSA, 2% sucrose, 0.1% PEG, 0.05% Tween-20 After 1 h, it was dried for later use; the concentrated solution of the colloidal gold-labeled bovine immunoglobulin antibody-a complex prepared by the preparation method of the colloidal gold-labeled bovine immunoglobulin antibody-a complex provided in Example 2 was 5-10 A...

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Abstract

The invention provides a bovine immunoglobulin (IgG) double-antibody sandwich colloidal gold immunochromatographic test strip, a preparation method, a kit and a detection method. The colloidal gold immunochromatography test strip is characterized by comprising a bottom plate, and a water absorption pad, a chromatographic membrane, a conjugate release pad and a sample pad which are arranged on thePVC bottom plate in an overlapping manner. The conjugate release pad is coated with a colloidal gold labeled bovine immunoglobulin antibody a compound, the chromatographic membrane is provided with adetection line and a quality control line, the detection line is coated with an anti-bovine immunoglobulin antibody b, and the quality control line is coated with goat anti-mouse IgG. The invention also provides a preparation method of the chromatographic test strip, a kit comprising the reagent strip, and a detection method of the kit. According to the technical scheme, the kit is prepared basedon the principles of double-antibody sandwich colloidal gold color development and gold-labeled immunoassay analyzer quantification, and can be used for rapidly and quantitatively detecting the content of bovine immunoglobulin (IgG).

Description

technical field [0001] The present invention relates to the field of detection reagent strips and kits, in particular to a bovine immunoglobulin (IgG) double-antibody sandwich colloidal gold immunochromatographic test strip, a method for preparing the reagent strip, and a kit comprising the reagent strip, And the detection method of the kit. Background technique [0002] Immunoglobulin Ig is an important part of the body's immune system. It is commonly found in the blood, milk, colostrum and lymph fluid of mammals. It is generally divided into five categories: IgG, IgA, IgD, IgM, and IgE. With the continuous improvement of the separation technology of bovine immunoglobulin, people's understanding of the biological function of bovine immunoglobulin is also deepening. It is rich in high-quality protein, vitamins and minerals and other nutrients, which can regulate the immune function of animals and enhance the immune function of animals. Body resistance, so it is more and mor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/577G01N33/558G01N33/532
CPCG01N33/532G01N33/558G01N33/577G01N33/587G01N33/6854
Inventor 邵俪黎赵启皓许丛文王宏陈宗伦蔡菁华戴仙兵黄佳娟钟文茵
Owner HANGZHOU BINGUO INFORMATION TECH CO LTD
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