Extraction method of white kidney bean amylase inhibitor

Abstract

The invention discloses an extraction method of a white kidney bean amylase inhibitor. The extraction method comprises white kidney bean starch extraction and amylase inhibitor extraction, wherein thewhite kidney bean starch extraction comprises the following steps of: S1, taking white kidney bean residues, adding pure water, performing stirring to enable the white kidney bean residues to disperse, adding a NaOH solution, performing extracting at a set temperature for a certain time, taking out precipitate, dispersing the precipitate in pure water, adjusting the pH value to 7.0, performing centrifuging, removing the clear liquid, washing lower starch with pure water, performing centrifuging, drying the filter cakes, performing crushing, and performing screening to obtain the white kidneybean starch; the amylase inhibitor extraction comprises the following steps of: S2, taking the white kidney bean starch obtained in the step S1, performing crushing and sieving, adding distilled water, performing continuous stirring at a constant temperature, performing centrifuging to obtain supernatant, performing ultrafiltration on a supernatant ultrafiltration membrane, freeze-drying trapped fluid, and performing crushing to obtain the white kidney bean amylase inhibitor. The yield of the white kidney bean amylase inhibitor is increased, white kidney beans are utilized as much as possible,and the starch utilization rate of the white kidney beans is increased.

Description

Technical field [0001] The invention belongs to the field of extraction technology of biological inhibitors, and particularly relates to a method for extracting white kidney bean amylase inhibitors. Background technique [0002] White kidney beans belong to the Leguminosae Papilionaceae subfamily, the Phaseolus vulgaris family, Phaseolus vulgaris, is a bean crop that is widely planted worldwide. Kidney bean grains contain 18%-21% protein and 40%-60% starch. With 1.6% to 2.15% fat, white kidney beans are not only rich in nutrition, but also have medicinal value. White kidney beans contain amylase inhibitors, which can inhibit the activity of amylase in saliva and pancreas, hinder the hydrolysis and digestion of carbohydrates in food, and are widely used in health foods such as weight loss and blood sugar control. [0003] At present, the use of white kidney beans to produce amylase inhibitors has been industrialized. The main component of amylase inhibitors is protein, which is low...

AI Technical Summary

Problems solved by technology

[0003] At present, the use of white kidney beans to produce amylase inhibitors has been industrialized. The main component of amylase inhibitors is protein, which has a low content. After its separation and extraction, the remaining bean dregs have a large proportion, and the bean dregs have a large water content, which is easy to spoil and deteriorate, and difficult to transport. , usually only used as feed

Therefore, if the bean dregs are not well developed and utilized, the economic benefits of production will be low and resources will be wasted.