Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Synthesis and characterization of the transport vector phlip-pei-pla and a method for studying its delivery to siRNA

A phlip-pei-pla and carrier technology, which is applied in drug combinations, pharmaceutical formulas, genetic material components, etc., can solve the problems of poor siRNA stability, low tumor targeting efficiency, and limited siRNA application, and achieves a simple and easy synthesis method , Improve serum stability and reduce cytotoxicity

Active Publication Date: 2022-03-18
SHANXI UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, naked siRNA has poor stability, is easily degraded by ribozymes in serum, has a short blood half-life, and has low transfection efficiency
Traditional gene delivery systems have low tumor targeting efficiency and cannot quickly and effectively escape endosomes, which severely limits the application of siRNA in tumor therapy
Polyethyleneimine (PEI) is a gold-standard polymer with excellent transfection efficacy, but its severe cytotoxicity and non-degradability hinder its therapeutic application as a gene delivery vehicle

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Synthesis and characterization of the transport vector phlip-pei-pla and a method for studying its delivery to siRNA
  • Synthesis and characterization of the transport vector phlip-pei-pla and a method for studying its delivery to siRNA
  • Synthesis and characterization of the transport vector phlip-pei-pla and a method for studying its delivery to siRNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0044]In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0045] The present invention provides a technical solution: the synthesis and characterization of the transport carrier pHLIP-PEI-PLA and its research method for the delivery of siRNA, including the following steps:

[0046] S1. Prepare reagents: 1640 liquid culture medium, mycoplasma-free fetal bovine serum, low pH insertion peptide pHLIP and FITC-labeled low pH insertion peptide (FITC-pHLIP), 4% paraformaldehyde fixative, anti-fluorescence attenuation mounting medium, DMSO, trypsin, penicillin-streptomycin mixture, siRNA targeting PKM2: 5'-CCAUAAUCGUCCUCACCCAA-3', negative control siRNA, Cy5-label...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention is applicable to the technical field of cancer treatment, and provides a research method for the synthesis and characterization of the transport carrier pHLIP-PEI-PLA and its delivery to siRNA, by first synthesizing the pHLIP-PEI-PLA copolymer, and measuring the pHLIP-PEI-PLA The complexing ability of the copolymer to siRNA, testing the serum stability of the pHLIP‑PEI‑PLA@siRNA complex; preparing the pHLIP‑PEI‑PLA@siRNA complex, and observing the cells’ response to the FITC‑pHLIP‑PEI‑PLA@siRNA complex Finally, the uptake of pHLIP‑PEI‑PLA@siRNA complexes by cells was analyzed by flow cytometry, so as to study the efficiency of pHLIP‑PEI‑PLA copolymers in delivering siRNA to tumor cells and the role of siRNA in tumor therapy. Applications provide a theoretical basis.

Description

technical field [0001] The invention belongs to the technical field of cancer treatment research, and in particular relates to the synthesis and characterization of a transport carrier pHLIP-PEI-PLA and a research method for its delivery to siRNA. Background technique [0002] Since the discovery of RNA interference technology (RNAi), drug research based on siRNA has made good progress, which provides a new idea for gene therapy of cancer. However, naked siRNA has poor stability, is easily degraded by ribozymes in serum, has a short blood half-life, and has low transfection efficiency. Traditional gene delivery systems have low tumor targeting efficiency and cannot quickly and effectively escape endosomes, which severely limits the application of siRNA in tumor therapy. Polyethyleneimine (PEI) is a gold-standard polymer with excellent transfection efficacy, but its severe cytotoxicity and non-degradability hinder its therapeutic application as a gene delivery vehicle. Con...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K31/713A61K47/64A61P35/00C08G81/00
CPCA61K31/713A61K47/64A61P35/00C08G81/00Y02A50/30
Inventor 丁国斌李卓玉孟雪马晓慧
Owner SHANXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com