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Nutritional composition, food/drink composition using nutritional composition, and modified milk powder using nutritional composition

A nutritional composition and a technology for bifidobacteria are applied in the field of nutritional compositions for probiotic proliferation, and can solve problems such as adverse effects on host health

Pending Publication Date: 2020-11-13
MORINAGA MILK IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Usually, the intestinal flora (Enteric flora) of healthy infants is dominated by bifidobacteria, but it gradually decreases with age. Due to the increase of spoilage bacteria such as Escherichia coli and Clostridium bacteria, it is harmful to the health of the host (human) produce adverse effects

Method used

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  • Nutritional composition, food/drink composition using nutritional composition, and modified milk powder using nutritional composition
  • Nutritional composition, food/drink composition using nutritional composition, and modified milk powder using nutritional composition
  • Nutritional composition, food/drink composition using nutritional composition, and modified milk powder using nutritional composition

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Experimental program
Comparison scheme
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Embodiment

[0206] Hereinafter, the present invention will be described in further detail based on examples. In addition, the Example described below is an example which shows the representative Example of this invention, and is not intended to interpret the scope of this invention narrowly.

[0207]

[0208] (1) Preparation of sugar solution

[0209] As an example of glucose (manufactured by NACALAI TESQUE, INC.), HMOs, 2'-FL (available from (≥95%) Funakoshi Co., Ltd., SIGMA Corporation), LNnT (manufactured by ELICITYL) were ion-exchanged A 10% solution was prepared in water and sterile filtered using Millex-GV (SLGV033RS).

[0210] (2) Preparation of Bacteria Liquid of Bifidobacterium and Lactic Acid Bacteria

[0211] Add Lactobacillus MRS Broth (BD) (0.05% L-cysteine ​​hydrochloride in Bifidobacterium bacteria) to the following Table 1 (bacteria belonging to Bifidobacterium bifidum) and the following Table 2 strains , inoculated with a 3% bacterial solution after the sterilization...

manufacture example

[0280] Production examples of nutritional compositions, pharmaceutical compositions, and food compositions are shown below.

[0281]

[0282] Bifidobacterium bifidum was added to 3 mL of MRS liquid medium and anaerobically cultured at 37° C. for 16 hours, then the culture solution was concentrated and freeze-dried to obtain a freeze-dried powder of the bacteria (minus). The powder containing Bifidobacterium bifidum was mixed with probiotics / human milk oligosaccharides (available from Funakoshi Co., Ltd.) / whey protein concentrate (Whey protein concentrate; WPC) and prebiotics (lactulose, cotton subsaccharides and galacto-oligosaccharides) were uniformly mixed to obtain a composition. 20 g of this composition was dissolved in 200 g of water to obtain a nutritional composition.

[0283]

[0284] Bifidobacterium bifidum was added to 3 mL of MRS liquid medium and anaerobically cultured at 37° C. for 16 hours, then the culture solution was concentrated and freeze-dried to obtai...

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Abstract

Provided is a technology that makes it possible to promote the growth, in the presence of human milk oligosaccharides (HMOs), of probiotics that have a low ability to assimilate HMOs. Provided is a nutritional composition that contains Bifidobacterium bifidum bacteria, at least one type of probiotic that has a low ability to assimilate human milk oligosaccharides, and human milk oligosaccharides.The nutritional composition is suitable for use in foods / drinks, drugs, quasi-drugs, feed, and the like.

Description

technical field [0001] The present invention relates to a nutritional composition capable of promoting the growth of probiotics with low assimilative ability of human milk oligosaccharides (hereinafter also referred to as "HMOs"). Background technique [0002] Bifidobacteria are known to be intestinal resident bacteria of mammals including humans, and have an action of suppressing the growth of pathogenic enterobacteria in the intestinal tract. [0003] Usually, the intestinal flora (Enteric flora) of healthy infants is dominated by bifidobacteria, but it gradually decreases with age. Due to the increase of spoilage bacteria such as Escherichia coli and Clostridium bacteria, it is harmful to the health of the host (human) produce adverse effects. Therefore, maintaining a bifidobacteria-dominated gut flora is particularly important for long-term health maintenance. [0004] Breast milk contains 10-20g / L of HMOs, which is said to be a mixture of more than 130 oligosaccharide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/135A23C9/127A23C9/13A23L33/125A61K31/702A61K35/744A61K35/745A61K35/747A61P3/02A61P43/00C12N1/20
CPCA23L33/125A23L33/135A61K31/702A61K35/745A61P3/02C12N1/20A23L33/40A61K35/20A23C9/1234A23C9/1307A23C9/203A23C9/16A61K2300/00A23L29/30A23C9/1232
Inventor 高桥幸子岩渊纪介
Owner MORINAGA MILK IND CO LTD
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